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- 应用范围
- Biochemical Assay (BCA)
- 特异性
- 100 ng/mL
- 产品特性
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Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 - 2,000 ng/mL) calf thymus DNA in 96-well plate assay.
Simple and high-throughput. The mix-and-read procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day.
Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay. - 组件
- Reagent: 50 mL. Standard: 1 mL 10 µg/mL calf thymus DNA.
- 试剂未包括
- Pipeting devices and accessories. 1 x TE buffer. Black 96-well plates (e.g. Corning) and fluorescence plate reader. Fluorescence spectrophotometer and fluorometric cuvets.
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Amyloid beta 1-40 (Abeta 1-40) ELISA KitAbeta 1-40 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 人 Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 小鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Direct Assays: plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices.
- 说明
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(1) For samples with unknown DNA concentration, pipet 1 μL sample and mix with 99 μL TE buffer. Make further serial 10-fold dilutions. Assay all diluted samples and choose dilutions at which the fluorescence intensity values fall within the linear calibration range to calculate sample DNA concentration.
(2). Calf thymus DNA serves as a standard for plant and animal DNA, because the AT content is conserved among most DNAs from these two species. For bacterial DNA, a different standard should be used that best matches the sample DNA content.
(3). Fluorescence intensity is half when binding to the same single-stranded DNA. Short single- stranded DNA pieces do not fluoresce with this dye. - 实验流程
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Procedure using 96-well plate:
1. Prepare 400 µL 2000 ng/mL Premix by mixing 80 µL Standard and 320 µL TE buffer (10mM Tris, 1mM EDTA, pH 7.4). Transfer 20 µL diluted standards and samples into wells of a black flat-bottom 96-well plate. Store standards at 4°C for future use.
2. Add 200 µL working reagent and tap lightly to mix.
3. Incubate 1 min at room temperature and read fluorescence emission at 440 - 460nm (peak 450nm, excitation at 340-370 nm).
Procedure using cuvette:
1. Transfer 100 µL diluted standards and samples to cuvets.
2. Add 1000 µL working reagent and tap lightly to mix.
3. Incubate 1 min at room temperature and read fluorescence intensity at 440 - 460nm (peak 450nm, excitation at 340-360 nm). - 试剂准备
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Bring reagents to room temperature before use.
- 结果分析
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Subtract blank fluorescence value (water, #8) from the standard values and plot F against standard DNA concentrations. Determine the slope using linear regression fitting.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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: "Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells." in: Bioelectromagnetics, Vol. 32, Issue 4, pp. 283-90, (2011) (PubMed).
: "Membrane-based cultures generate scaffold-free neocartilage in vitro: influence of growth factors." in: Tissue engineering. Part A, Vol. 16, Issue 2, pp. 513-21, (2010) (PubMed).
: "Ambivalent effects of compound C (dorsomorphin) on inflammatory response in LPS-stimulated rat primary microglial cultures." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 381, Issue 1, pp. 41-57, (2010) (PubMed).
: "Metformin has adenosine-monophosphate activated protein kinase (AMPK)-independent effects on LPS-stimulated rat primary microglial cultures." in: Pharmacological reports : PR, Vol. 62, Issue 5, pp. 827-48, (2010) (PubMed).
: "Structure activity relationships and differential interactions and functional activity of various equine estrogens mediated via estrogen receptors (ERs) ERalpha and ERbeta." in: Endocrinology, Vol. 149, Issue 10, pp. 4857-70, (2008) (PubMed).
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: "Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells." in: Bioelectromagnetics, Vol. 32, Issue 4, pp. 283-90, (2011) (PubMed).
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- 背景
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Quantitative determination of DNA by fluorimetric (340nm/450nm) method.
Procedure: 5 min.
DNA quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260 nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling. Simple, direct and automation-ready procedures for measuring DNA concentration are very desirable. This DNA assay kit is designed to accurately measure nanogram quantities of plasmid DNA, cDNA, DNA following polymerase chain reaction and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double-stranded DNA. The fluorescence intensity, measured at 450nm (exc = 350nm), is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
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