Ascorbic Acid Assay Kit
BCA
Cell Culture Supernatant, Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Urine
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- 抗原 See all Vitamin C products
- Vitamin C (Ascorbic Acid (Vitamin C))
- 应用范围
- Biochemical Assay (BCA)
- 样品类型
- Cell Culture Supernatant, Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Urine
- 产品特性
- Use 20 µL samples. Linear detection range: colorimetric assay 6 to 1000 µM, fluorimetric assay 1 to 100 µM ascorbic acid.
- 组件
- Assay Buffer: 10 mL. Enzyme Mix: 120 µL. Dye Reagent: 120 µL. Standard: 400 µL 10 mM ascorbic acid.
- 试剂未包括
- Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.
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Ascorbic Acid (Vitamin C) ELISA Kit
适用: Various Species Colorimetric Competition ELISA 493.8 ng/mL - 40000 ng/mL Plasma, Serum
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- 应用备注
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Assays: ascorbic acid in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.
Drug Discovery/Pharmacology: effects of drugs on ascorbic acid metabolism. - 结果分析
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Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.
Conversions: 1 mM ascorbic acid equals 17.6 mg/dL, 0.0176% or 176 ppm. - 限制
- 仅限研究用
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- 储存条件
- -20 °C
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- 抗原
- Vitamin C (Ascorbic Acid (Vitamin C))
- 别名
- Ascorbic Acid (Vitamin C 产品)
- 物质类
- Chemical
- 背景
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Quantitative determination of ascorbic acid by colorimetric (570nm) or fluorimetric (530nm/590nm) methods.
Procedure: 10 min.
Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. This method provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.
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