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- 抗原 See all IgG products
- IgG
- 片段
- Fab fragment
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宿主
- 小鼠
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunohistochemistry (IHC)
- Supplier Product No.
- 010-0105
- Supplier
- Rockland
- 原理
- Mouse IgG Fab Fragment
- 交叉反应 （详细）
- Mouse IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG, anti-Mouse IgG F(ab')2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti- Papain.
- 产品特性
- Mouse Immunoglobulin Fab, F(ab), Fragment antigen-binding
- 纯化方法
- Mouse IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 亚型
- IgG
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Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype control (PE)FACS, ELISA Polyclonal IgG PE
Goat IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Mouse IgG Fab Fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
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