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- 抗原 See all IgG products
- IgG
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宿主
- Human
- 标记
- Biotin
- 应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Supplier Product No.
- 009-0602
- Supplier
- Rockland
- 原理
- Human IgG Whole Molecule Biotin Conjugated
- 交叉反应 （详细）
- Human IgG whole molecule Biotin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG and anti-Human Serum.
- 产品特性
- Human IgG Biotin conjugation, Human immunoglobulin G, Vitamin H, coenzyme R
- 纯化方法
- Human IgG whole molecule Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 亚型
- IgG
- Labeling Ratio
- 10-20
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Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype control (PE)FACS, ELISA Polyclonal IgG PE
Goat IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:500
Application Note: Human IgG whole molecule Biotin conjugated has been tested in ELISA and can be utilized as a control reagent in both Western Blotting and ELISA format experiments.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:5000 - 1:50,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Human IgG whole molecule Biotin conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This Human IgG whole molecule is conjugated to biotin (Vitamin H), a small biomolecule that has a large affinity for avidin and streptavidin.
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