大鼠 IgG2a isotype control (FITC)
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- 抗原 See all IgG2a products
- IgG2a
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宿主
- 大鼠
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克隆类型
- 单克隆
- 标记
- FITC
- 应用范围
- Flow Cytometry (FACS)
- 原理
- Rat IgG2a Isotype Control FITC
- 特异性
- This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
- 纯化方法
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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克隆位点
- RTG2A1-1
- 亚型
- IgG2a
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- 应用备注
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- 抗原
- IgG2a
- Abstract
- IgG2a 产品
- 别名
- gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
- 物质类
- Antibody
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