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- 抗原 See all IgM products
- IgM
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宿主
- Human
- 应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), SDS-PAGE (SDS)
- Supplier Product No.
- 009-0107-0001
- Supplier
- Rockland
- 原理
- Human IgM (myeloma) Whole Molecule
- 交叉反应 （详细）
- Human IgM (myeloma) whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgM (μ chain specific). No reaction was observed against anti-Human IgG F(c). Some light chain cross-reactivity will occur with anti-Human IgG.
- 产品特性
- Human immunoglobulin M, Human IgM whole molecule, Human IgM myeloma whole molecule, Hu IgM WM
- 纯化方法
- Human IgM (myeloma) whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 亚型
- IgM
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Mouse IgM isotype control (FITC)
IsoC, FACS, IF IgM FITC
Mouse IgM isotype controlIsoC, FACS IgM unconjugated
Mouse IgM isotype control (FITC)IsoC, FACS, IF IgM FITC
Mouse IgM isotype control (PE)IsoC, FACS, IF IgM PE
Mouse IgM isotype control (PE-Cy5)IsoC, FACS, IF IgM PE-Cy5
Mouse IgM isotype control (PE)IsoC, FACS, IF IgM PE
Mouse IgM isotype control (PE-Cy5)IsoC, FACS, IF IgM PE-Cy5
Mouse IgM isotype control (APC)IsoC, FACS, IF IgM APC
Mouse IgM isotype controlIsoC, FACS IgM unconjugated
Mouse IgM isotype control (APC)IsoC, FACS, IF IgM APC
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Human IgM whole molecule has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.1 mg/mL
- 缓冲液
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Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
- Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
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