Infliximab ELISA 试剂盒
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- 抗原
- Infliximab
- 适用
- 人, 小鼠, 大鼠, 猴
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.2-6 μg/mL
- 最低检测浓度
- 0.2 μg/mL
- 应用范围
- ELISA
- 原理
- Enzyme immunoassay for the quantitative determination of free Infliximab in human serum and plasma.
- 品牌
- ImmunoGuide®
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- Infliximab (Remicade®, Remsima®)
- 灵敏度
- 10 ng/mL
- 产品特性
- This kit measures the free drug concentration.
- 组件
- plate, standards, assay buffer, conjugate, TMB, HCl, wash buffer
- 试剂未包括
- Normal lab equipment for performing ELISA assays
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- 应用备注
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- Before performing the assay, samples and assay kit should be brought to room temperature (about 30 minutes beforehand) and ensure the homogeneity of the solution.
- All Standards should be run with each series of unknown samples.
- Standards should be subject to the same manipulations and incubation times as the samples being tested.
- All steps of the test should be completed without interruption. 6
- Use new disposable plastic pipette tips for each reagent, standard or specimen in order to avoid cross contamination.
- 说明
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0.2 - 6 µg/mL (in case of the recommended sample dilution factor)
10 ng/ml (sensitivity in the well) - 样本量
- 20 μL
- 实验时间
- 1.5 h
- 板类型
- Pre-coated
- 实验流程
- This is a sandwich type ELISA. Standards and diluted samples (serum or plasma) are incubated in the microtitre plate coated with recombinant human TNF-a (rh TNF-a). After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-human IgG monoclonal antibody is added and binds to the Fc part of Infliximab pre-captured by the rh TNF-a on the surface of the wells. Following incubation, the wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Infliximab in the sample or standard. Results of samples can be determined directly by using the standard curve.
- 试剂准备
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Just the wash buffer has to be prepared by diluting the stock solution 1:20. All other reagents are ready to use.
- 样品收集
- Normal serum or plasma collection
- 样品制备
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dilute the samples 1:20 with assay buffer
- 实验流程
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Pipette 100 µL of assay buffer into each of the wells.
Pipette 20 µL of each standard and diluted samples.
Incubate 30 min at room temperature (RT).
Aspirate or decant the incubation solution.
Wash the plate 3 x 300 μL of diluted wash buffer per well.
Pipette 100 μL of enzyme conjugate (HRP-anti human IgG mAb).
Shake plate carefully. Incubate 30 min at RT.
Wash the plate 3 x 300 μL of diluted wash buffer per well.
Pipette 100 µL TMB substrate solution into each well.
Incubate 15 min at RT.
Add 100 µL of stop solution.
Measure optical density (OD) at 450 nm. - 结果分析
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The results are read from a standard curve.
- 实验精密度
- < 10%
- 限制
- 仅限研究用
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- 缓冲液
- < 15mM NaN3
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
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The kit is shipped at ambient temperature and should be stored at 2-8°C.
Keep away from heat or direct sun light.
The storage and stability of specimen and prepared reagents is stated in the corresponding chapters.
The microtiter strips are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2-8°C. - 有效期
- 24 months
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- 抗原
- Infliximab
- 物质类
- Antibody
- 背景
- The drug Infliximab (trade name Remicade® and Remsima®) is a chimeric monoclonal antibody and used to treat auto-immune disorders
- 分子量
- 144 kDa
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