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Protease S. aureus

1 mg/mL
产品编号 ABIN5706431
发货至: 中国
  • 纯化方法
    Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay.
    Biological Activity Comment
    880 units/mg
  • 应用备注

    Application Note: Protease S. aureus has shown that one unit causes a change of 0.001 A280 nm per minute at 37° C at  pH 7.8 using casein as a substrate. Specific conditions for reactivity should be optimized by end user.

    说明

    Synonyms: Glutamyl endopeptidase,Endoproteinase Glu-C, Staphylococcal serine proteinase, V8 protease, V8 proteinase

    Background: Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum  pH 's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay. Protease S. aureus is ideal for investigators involved in enzyme and infectious diease research.

    Gene Name: sspA

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1 mg/mL
    缓冲液

    Buffer: None

    Stabilizer: None

    储存液
    Without preservative
    储存条件
    4 °C,-20 °C
    有效期
    12 months
  • 基因ID
    1280
    UniProt
    P0C1U8
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