Antibody Affinity Maturation

Affinity maturation of antibodies can improve the function and performance of your antibody. We can use various approaches to alter the binding characteristics of an antibody through mutations introduced into the antibody variable region. By altering the antibody sequence, the association and/or dissociation rate of the antibody can be modulated to enhance or weaken the interaction with the antigen.

Our Approach to Affinity Maturation

Affinity maturation can be used to enhance the binding characteristics of your antibody via various affinity maturation approaches. Depending on the present affinity of your antibody, binding affinity can be often improved by at least 10x. For some applications, a weaker binding affinity may be desired and this can be achieved as well where needed. Methods used for the generation of sub-libraries, which include error-prone PCR for the introduction of sequence substitutions or the total synthesis and randomization of CDRs in your antibody, are implemented to seek clones with altered binding characteristics and kinetics such as on- and off-rates. Talk to our experts to explore how Rockland can help improve your antibody for better performance.