C2ORF78 Protein (AA 1-922) (Strep Tag)
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- 抗原
- C2ORF78 (Chromosome 2 Open Reading Frame 78 (C2ORF78))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-922
- 宿主
- 人
- 资源
- Tobacco (Nicotiana tabacum)
- 标记
- This C2ORF78 protein is labelled with Strep Tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
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MHWLASATQT SASIVSSSLL SAVDVSSSLT MSEYFQNTSL PGTANSRQFS LPVVSNAAFL TGSISNFSRA SAPAISSAWL QPSASGTSFQ PLMGSAYLYQ HSSTTMLSGV TGQSHICTSA ASYPGVFEWD STASTVKKSS SLRDFTVTVI DQNTAVSSMS MTAQYYKTSD TNTMVPLYPS LSASLVQGTL TQIPNQQGHN LSLPCQIGSQ VYYYNQGTLG PQLSCLQSYG SVSYTGYRAS AHQPEMVMVL KEVQPTNVLP PVSTSGMYYS VSSQPITETS VQVMETSLGM DTSLGLQSPS QTFCLPQTPE FSKSFSSRNT QTLESNPSPE LGDISITPVQ SPTNLLTLSP APSQEKNENE NLDEIKTNLS KPLDVHQILI GNQDPPLLPV EIPDIHPLLA CIDPLGQEEQ PGSENANLRN KSLSLEDQGI FENGIESSSD LADITTWVED TYLPPIFSSL QDLDQPESPS AKKAKDTSAI KVNQVQEKSC VIKGHSDQVR KNKHKASEPI QGAPKAKIQP KNPECLLERE VVVGSATVSN SASVNKAKHS SNKPHKAASS RISKTKSHGQ EKTKGNRKNS SKKSEESKQS GKKVKVEEKQ TIPNMKRKKN QPELSQKTLK KPRSSLGMHM LESVQVFHAL GKKIDMKTGF SSSRTLGSSS NTQNRQPFPA LKPWLDIQHE GKGPEKIQVK AQKLDGSAEK ECTSPSHSEL PPPGKVKLIP LPFLTLDQPQ ARHVSRRPNP LASRRPAVAY PARPDSTNSA QSNAVNPSRP APTNTSLTGP ATPAQPISAK ATQPSSANPT QPTVPQSAAS RPSAYKTSSC SSLQREPVST AVTSLRSLPK PQNQFLIQDF SLQPRPWRKP TVPEPVMSTP ITEEQRPERE AMKRKAQQER ENAAKYTSLG KVQFFIERER DMEIAEYYGY TI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- 纯度
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- C2ORF78 (Chromosome 2 Open Reading Frame 78 (C2ORF78))
- 别名
- C2orf78
- 别名
- COG5373 Protein, hCG1989538 Protein, chromosome 2 open reading frame 78 Protein, C2orf78 Protein
- 背景
- Uncharacterized protein C2orf78
- 分子量
- 100.2 kDa
- UniProt
- A6NCI8
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