Quick Overview for TMB ELISA Peroxidase Substrate (ABIN925629)
应用范围
ELISA
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Supplier Product No.
- tmbe-100
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Supplier
- Rockland
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产品特性
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TMB ELISA Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction can be halted with acid to change the TMB to yellow.
Synonyms: 3,3’,5,5’-Tetramethylbenzidine; TMB-E; TMBE
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS (pH 7.5)BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS pH 7.210 X
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
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应用备注
- TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 X
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储存条件
- 4 °C
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