20X SSC pH 7.0
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- 纯化方法
- 20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC Treated was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. 20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC Treated was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
- 过滤
- Aseptic filtered
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
ELISA Coating StabilizerELISA 1 X
TMB ELISA Peroxidase SubstrateELISA 1 X
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- 应用备注
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Application Note: 20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC Treated is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC Treated contains 3.0 M Sodium Chloride, 0.3 M Sodium Citrate adjusted to pH 7.0. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water treated with diethyl pyrocarbonate (DEPC).
- 说明
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Synonyms: Saline-sodium citrate, sodium chloride sodium citrate, SSC
Background: Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridization process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridization, or DNA microarrays). SSC is used to help control the stringency of hybridization in these steps.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 20 X
- 缓冲液
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Buffer: See application note.
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- RT
- 有效期
- 6 months
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