Loading Buffer (5X)
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- 产品特性
- Loading buffer for separation and visualization proteins with SDS-PAGE and Western Blot analysis.
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- 应用备注
- Sample can be prepared under reducing conditions with the addition of a reducing agent such as beta-mercaptoethanol or DTT. Dilute to 1X in sample. Do not heat prior to loading on gel. For reducing buffer, add 5 % (v/v) beta-mercaptoethanol or 1 mM DTT to 1X loading buffer and sample. Boil sample/loading buffer for 5 min. Do not store buffer with reducing agent, add fresh prior to each use.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 储存条件
- -20 °C
- 储存方法
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Short Term Storage: +20°C
Long Term Storage: +20°C
Stable for at least 2 years after receipt when stored at +20°C. - 有效期
- 24 months
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