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- 应用范围
- Intracellular Flow Cytometry (ICFC)
- 品牌
- BD Phosflow™
- 产品特性
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BD™ Phosflow Perm/Wash Buffer I is intended to be used for the intracellular staining of post-translationally modified signaling proteins.
BD™ Phosflow Perm/Wash Buffer I is used to permeabilize cells and to serve as an antibody diluent and cell wash buffer. It is optimized for use with the BD™ Phosflow brand of intracellular phosphorylated signaling protein-specific antibodies. BD™ Phosflow Perm/Wash Buffer I is provided as a 10X concentrated solution containing FBS and saponin. The presence of a small amount of precipitate may be observable and will not affect the performance of the buffer. Because saponin-mediated cell permeabilization is a reversible process, it is important to keep the cells in the presence of saponin during intracellular staining.
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
ELISA Coating StabilizerELISA 1 X
TMB ELISA Peroxidase SubstrateELISA 1 X
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing saponin, fetal bovine serum and ≤ 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. Do not freeze.
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: "Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 55, Issue 2, pp. 61-70, (2003) (PubMed).
: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Detection of intracellular cytokines by flow cytometry." in: Journal of immunological methods, Vol. 159, Issue 1-2, pp. 197-207, (1993) (PubMed).
: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, (1991) (PubMed).
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: "Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 55, Issue 2, pp. 61-70, (2003) (PubMed).
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