(12 references)-
- 应用范围
- Purification (Purif)
- 特异性
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High binding capacity: The kit can handle over 20 mg 6xHis-tagged protein/mL (CV).
Simple purification procedure under both native and denaturing conditions.
Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH 14 for short periods.
Resilient: If properly regenerated, the resin can be reused multiple times. - 产品特性
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High Affinity Ni-Charged Resin is an 4 % cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins. High Affinity Ni-Charged Resin is available in 10, 25 and 500 mL lab packs.
Total Volume: 10 mL settled resin (20 mL 50 % slurry) 25 mL settled resin (50 mL 50 % slurry) 500 mL settled resin (1000 mL 50 % slurry) - Bead Ligand
- Nickel ions
- Bead Matrix
- Agarose beads
- Bead Size
- 90 µm
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Magnetic Concanavalin A Beads (Agarose)
CUT&RUN, CUT&Tag, Sep Concanavalin A Magnetic Agarose beads 30 μm
Ni-NTA MagBeadsPull-Down, Purif, Sep Ni-NTA 25 µm
Ni-NTA AgarosePurif, Sep Ni-NTA 40 µm
Rho1D4 AgarosePurif, Sep Rho1D4 40 µm
BFP-CatcherRIP, Co-IP, IP, Purif, ChIP Antibody Agarose beads 90 μm
INDIGO Ni-AgarosePurif Ni-INDIGO Agarose beads 100 μm
MagSi-proteomics C18 beadsPurif, Sep, Conc, PD C18 alkyl group Magnetic Silica particles Bead size: 1.2 µm
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- 说明
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For ambient pressure use only.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 储存条件
- 4 °C
- 储存方法
- Store the product between 2°C and 8°C.
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: "A single whey acidic protein domain containing protein (SWD) inhibits bacteria invasion and dissemination in shrimp Marsupenaeus japonicus." in: Fish & shellfish immunology, Vol. 35, Issue 2, pp. 310-8, (2013) (PubMed).
: "A fibrinogen-related protein (TfFREP2) gene involving in the immune response of Trachidermus fasciatus against Vibrio anguillarum." in: Fish & shellfish immunology, Vol. 35, Issue 6, pp. 1988-92, (2013) (PubMed).
: "Apoptotic effect of Vibrio vulnificus cytolysin on A549 human lung adenocarcinoma cells." in: Molecular medicine reports, Vol. 5, Issue 3, pp. 668-74, (2012) (PubMed).
: "ErbB-2 signals through Plexin-B1 to promote breast cancer metastasis." in: The Journal of clinical investigation, Vol. 122, Issue 4, pp. 1296-305, (2012) (PubMed).
: "Expression of human globular adiponectin-glucagon-like peptide-1 analog fusion protein and its assay of glucose-lowering effect in vivo." in: International journal of medical sciences, Vol. 8, Issue 3, pp. 203-9, (2011) (PubMed).
: "A monoclonal antibody specific to human myxovirus resistance protein A." in: Hybridoma (2005), Vol. 29, Issue 1, pp. 31-6, (2010) (PubMed).
: "Vaccination with combination of Fit3L and RANTES in a DNA prime-protein boost regimen elicits strong cell-mediated immunity and antitumor effect." in: Vaccine, Vol. 27, Issue 7, pp. 1111-8, (2009) (PubMed).
: "Vibrio vulnificus cytolysin induces apoptosis in HUVEC, SGC-7901 and SMMC-7721 cells via caspase-9/3-dependent pathway." in: Microbial pathogenesis, Vol. 46, Issue 4, pp. 194-200, (2009) (PubMed).
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: "Characterization of the insertase BamA in three different membrane mimetics by solution NMR spectroscopy." in: Journal of biomolecular NMR, Vol. 61, Issue 3-4, pp. 333-45, (2015) (PubMed).
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