Protein G is used for immunoprecipitation and purification of monoclonal and polyclonal antibodies.
特异性
Sepharose™ Protein G binds to all IgG subclasses from human, monkey, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
产品特性
Protein G is prepared by covalently coupling recombinant Protein G to 6% crosslinked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG, resulting in IgG binding greater than 10 mg of human IgG per cc of slurry. Protein G is used for immunoprecipitation and purification of monoclonal and polyclonal antibodies. This product shows stability within pH range of 2 to 10, is high flow rate capable, and has low falling off of rProtein G.
Protein G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.
限制
仅限研究用
状态
Liquid
缓冲液
Supplied in 20% Ethanol/Water
储存条件
4 °C
储存方法
Store SEPHAROSE PROTEIN G at 4 °C prior to opening. DO NOT FREEZE.