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人 RTK Phosphorylation Array G1

适用: 人 AA Fluorometric Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Glass Slide
产品编号 ABIN625613
发货至: 中国
  • 适用
    检测方法
    Fluorometric
    实验类型
    Sandwich ELISA
    应用范围
    Antibody Array (AA)
    原理
    G-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Human RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
    品牌
    RayBio®
    样品类型
    Plasma, Cell Culture Supernatant, Serum, Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    特异性
    ABL1, ACK, ALK-1, Axl, Blk, BMX, Btk, Csk, Dtk, EGFR, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ErbB2, ErbB3, ErbB4, FAK, FER, FGFR1, FGFR2, FGFR2 (alpha isoform), Fgr, FRK, Fyn, Hck, HGFR, IGF-1 R, Insulin R (CD220), Itk, JAK1, JAK2, JAK3, LCK, LTK, Lyn, MATK, M-CSF R, MUSK, NGFR (TNFRSF16), PDGFRA, PDGFRB, PYK2, RET, ROR1, ROR2, ROS, RYK, SCF R (CD117/c-kit), SRMS, SYK, Tec, Tie-1, Tie-2, TNK1, TRKB, TXK, Tyk2, TYRO10 (DDR2/TKT), VEGFR2, VEGFR3
    产品特性
    • High sensitivity and specificity
    • Low sample volume (10-100 μL per array)
    • Large dynamic range of detection
    • Compatible with most sample types
    • Test 4 or 8 samples on each slide
    • Suitable for high-throughput assays
    组件
    Cytokine Antibody Array glass slide (4 or 8 arrays per slide)
    Biotinylated Detection Antibodies
    Streptavidin-conjugated HiLytePlus™ 555 Fluor
    Blocking Buffer
    20X Wash Buffer I
    20X Wash Buffer II
    2X Cell Lysis Buffer
    G-Series Antibody Array accessories
    Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film
    试剂未包括
    Small plastic boxes or containers
    Pipettors, pipette tips and other common lab consumables
    Orbital shaker or oscillating rocker
    Aluminum foil
    Gene microarray scanner or similar laser fluorescence scanner
  • 应用备注
    Completely cover array area with sample or buffer during incubation. Avoid foaming during incubation steps. Perform all incubation and wash steps under gentle rocking or rotation. Cover the incubation chamber with adhesive film during incubation, particularly when incubation is more than 2 hours or <70 μL of sample or reagent is used. Several incubation steps such as step 6 (blocking), step 7 (sample incubation), step 10 (detection antibody incubation), or step 13 (Cy3 equivalent dyestreptavidin incubation) may be done overnight at 4 °C. Please make sure to cover the incubation chamber tightly to prevent evaporation.
    说明

    The G-Series arrays feature fluorescent signal detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection.
    All G-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.

    样本量
    100 μL
    实验时间
    6 h
    板类型
    Glass Slide
    实验流程
    1. Dry the glass slide
    2. Block array surface
    3. Incubate with Sample
    4. Incubate with Biotinylated Detection Antibody Cocktail
    5. Incubate with Streptavidin-Conjugated Fluor
    6. Disassemble the glass slide
    7. Scan with a gene microarray laser scanner
    8. Perform densitometry and analysis
    试剂准备
    1. Protease Inhibitor Cocktail: Briefly spin down the Protease Inhibitor Cocktail tube before use. Add 60 µl of 1x Lysis Buffer into the vial to prepare a 100X Protease Inhibitor Cocktail. 2. Phosphatase Inhibitor Cocktail Set II: Briefly spin down the Phosphatase Inhibitor Cocktail Set II tube before use. Add 180 µl of 1X Lysis Buffer into the vial to prepare 25X Phosphatase Inhibitor Cocktail Set II Concentrate . Dissolve the powder thoroughly by a gentle mix. 3. 2X Cell Lysis Buffer: Cell lysis buffer should be diluted 2-fold with deionized or distilled water. Add 20 µl of Protease Inhibitor Cocktail Concentrate and 80 µl of Phosphatase Inhibitor Cocktail Set II Concentrate into 1.9 ml of 1X Lysis Buffer before use. Mix well. 4. 20X Washing Buffer I or II : If the 20X Wash Concentrate contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 25 ml of Wash Buffer Concentrate into deionized or distilled water to yield 500 ml of 1X Wash Buffer. 5. Biotinylated anti-Phosphotyrosine: Briefly spin the Detection Antibody tube before use. Add 65 µl of Blocking Buffer into the tube to prepare a Biotinylated Anti-phosphotyrosine Concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). Add 30 µl of Detection Antibody Concentrate into a tube with 570 µl of Blocking Buffer. Mix gently to prepare 1X Biotinylated Anti-phosphotyrosine. 6. Fluorescent dye-Conjugated Streptavidin (cy3 equivalent): briefly spin the Fluorescent dye-Conjugated Streptavidin before use. Add 50 µl of Blocking Buffer into the tube to prepare a Streptavidin Concentrate. Pipette up and down to mix gently. Add 10 µl of Streptavidin Concentrate into a tube with 1 ml of Blocking Buffer. Mix gently to prepare 1X Streptavidin solution.
    样品制备

    Use serum-free conditioned media if possible. If serum-containing conditioned media is required, it is highly recommended that complete medium be used as a control since many types of sera contains cytokines. We recommend the following parameters for your samples: 50 to 100 µl of original or diluted serum, plasma, cell culture media, or other body fluid, or 50-500 µg/ml of protein for cell and tissue lysates. If you experience high background or if the fluorescent signal intensities exceed the detection range, further dilution of your sample is recommended.

    实验流程

    Take out the glass slide from the box, and let it equilibrate to room temperature inside the sealed plastic bag for 20-30 minutes. Remove slide from the plastic bag, peel off the cover film, and let it air dry for another 1-2 hours.

    Blocking & Incubation
    1. Add 100 µl Sample Diluent into each well and incubate at room temperature for 30 minutes to block slides.
    2. Decant buffer from each well. Add 100 µl of sample to each well. Incubate arrays at room temperature for 1-2 hour.
    3. Decant the samples from each well, and wash 5 times (5 min each) with 150 µl of 1X Wash Buffer I at room temperature with gentle shaking. Completely remove wash buffer in each wash step. Dilute 20x Wash Buffer I with H2O.
    4. Decant the 1x Wash Buffer I from each well, wash 2 times (5 min each) with 150 µl of 1X Wash Buffer II at room temperature with gentle shaking.Completely remove wash buffer in each wash step. Dilute 20X Wash Buffer II with H2O.

    Incubation with Biotinylated Antibody Cocktail & Wash
    5. Reconstitute the detection antibody by adding 1.4 ml of Sample Diluent to the tube. Spin briefly.
    6. Add 80 µl of the detection antibody cocktail to each well. Incubate at room temperature for 1-2 hour.
    7. Decant the samples from each well, and wash 5 times (5 mins each) with 150 µl of 1X Wash Buffer I and then 2 times with 150 µl of 1x Wash Buffer II at room temperature with gentle shaking. Completely remove wash buffer in each wash step.

    Incubation with Cy3 Equivalent Dye-Streptavidin & Wash
    8. After briefly spinning down, add 1.4 ml of Sample Diluent to Cy3 equivalent dye-conjugated streptavidin tube. Mix gently.
    9. Add 80 µl of Cy3 equivalent dye-conjugated streptavidin to each well. Cover the device with aluminum foil to avoid exposure to light or incubate in dark room. Incubate at room temperature for 1 hour.
    10. Decant the samples from each well, and wash 5 times (5 mins each) with 150 µl of 1X Wash Buffer I at room temperature with gentle shaking. Completely remove wash buffer in each wash step.

    Fluorescence Detection
    11. Disassemble the device by pushing clips outward from the slide side. Carefully remove the slide from the gasket.
    12. Place the slide in the Slide Washer/Dryer (a 4-slide holder/centrifuge tube), add enough 1x Wash Buffer I (about 30 ml) to cover the whole slide, and then gently shake at room temperature for 15 minutes. Decant Wash Buffer I. Wash with 1x Wash Buffer II (about 30 ml) and gently shake at room temperature for 5 minutes.
    13. Remove water droplets completely by gently applying suction with a pipette to remove water droplets. Do not touch the array, only the sides.
    14. Imaging: The signals can be visualized through use of a laser scanner equipped with a Cy3 wavelength (green channel) such as Axon GenePix.

    结果分析

    Data extraction can be done using the GAL file that is specific for this array along with the microarray analysis software (GenePix, ScanArray Express, ArrayVision, MicroVigene, etc.).

    限制
    仅限研究用
  • 注意事项
    Do not touch the surface of the slides, as the microarray slides are very sensitive. Hold the slides by the edges only. Handle all buffers and slides with powder free gloves. Handle glass slide/s in clean environment. The G-Series slides do not have bar codes. To help distinguish one slide from another, transcribe the slide serial number from the slide bag to the back of the slide with a fine point permanent marker. Please write the number on the very bottom edge of the slide, taking care to avoid writing on the array well areas.
    储存条件
    -20 °C
    储存方法
    For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
    有效期
    6 months
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    Cui, Xie, Luan, Zhou, Han: "Identification of the receptor tyrosine kinases (RTKs)-oriented functional targets of miR-206 by an antibody-based protein array." in: FEBS letters, Vol. 589, Issue 16, pp. 2131-5, (2015) (PubMed).

    Zhang, Pelech: "Using protein microarrays to study phosphorylation-mediated signal transduction." in: Seminars in cell & developmental biology, Vol. 23, Issue 8, pp. 872-82, (2012) (PubMed).

    Qi, Cooke, Stejskal, Riley, Croce, Saldanha, Bearss, Mahadevan: "MP470, a novel receptor tyrosine kinase inhibitor, in combination with Erlotinib inhibits the HER family/PI3K/Akt pathway and tumor growth in prostate cancer." in: BMC cancer, Vol. 9, pp. 142, (2009) (PubMed).

    Heiss: "[On the problem of induced abortion]." in: Geburtshilfe und Frauenheilkunde, Vol. 25, Issue 9, pp. 862-83 (PubMed).

  • 背景
    Protein phosphorylation plays an unusually prominent role in cell signaling, development and growth.
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