CUT&RUN Antibodies

CUT&RUN overcomes various downfalls of ChIP-Seq with improved workflow. CUT&RUN is simple to perform and is inherently robust. Our CUT&RUN antibodies against histone post-translational modifications and other targets have been tested very thoroughly to ensure highest reliability and best performance.

To identify best-in-class antibodies for use in CUT&RUN assays, antibodies-online has developed a rigorous validation process working with customers and manufacturers. This allows us to provide you with an ever-increasing portfolio of reliable antibodies for this innovative method. Therefore, it is best to check our websites regularly.

Tips for Antibody Selection

The successful execution of cleavage under target and release using nuclease (CUT&RUN) hinges on antibody selection. A factor- or histone-specific antibody is bound to chromatin in situ followed by binding to the antibody of a protein A-micrococcal nuclease (pA-MNase) fusion. As is the case with ChIP, the success depends in large part on the affinity of the antibody for its target and its specificity under the conditions used for binding. We compiled a list of primary and secondary antibodies suitable for CUT&RUN down below. Addtionally, antibodies-online supports the validation of antibodies for CUT&RUN. You can participate in our independent validation initiative (IVI). Propose and perform a validation experiment and get a full refund on the validated antibody. Please contact us, if you are interested in validating one of our CUT&RUN products!

Antibodies validated for CUT&RUN Assays

Controls for CUT&RUN

Our secondary antibody ABIN101961 was used as negative control and is referenced in the original paper on the CUT&RUN method (Peter J. Skene and Steven Henikoff, 2018). Use it in your CUT&RUN assays as well:

Learn more about CUT&RUN

• CUT&RUN - an Introduction
• CUT&RUN Combo - Everything you need for CUT&RUN
• CUT&Tag - Cleavage Under Targets and Tagmentation