CUT&RUN overcomes various downfalls of ChIP-Seq with improved workflow. CUT&RUN is simple to perform and is inherently robust. Our CUT&RUN antibodies against histone post-translational modifications and other targets have been tested very thoroughly to ensure highest reliability and best performance.
To identify best-in-class antibodies for use in CUT&RUN assays, antibodies-online has developed a rigorous validation process working with customers and manufacturers. This allows us to provide you with an ever-increasing portfolio of reliable antibodies for this innovative method. Therefore, it is best to check our websites regularly.
The successful execution of cleavage under target and release using nuclease (CUT&RUN) hinges on antibody selection.
A factor- or histone-specific antibody is bound to chromatin in situ followed by binding to the antibody of a protein A-micrococcal nuclease (pA-MNase) fusion.
As is the case with ChIP, the success depends in large part on the affinity of the antibody for its target and its specificity under the conditions used for binding.
We compiled a list of primary and secondary antibodies suitable for CUT&RUN down below.
Addtionally, antibodies-online supports the validation of antibodies for CUT&RUN.
You can participate in our independent validation initiative (IVI).
Propose and perform a validation experiment and get a full refund on the validated antibody. Please contact us, if you are interested in validating one of our CUT&RUN products!
Our secondary antibody ABIN101961 was used as negative control and is referenced in the original paper on the CUT&RUN method (Peter J. Skene and Steven Henikoff, 2018). Use it in your CUT&RUN assays as well: