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Polyclonal Antibodies

Polyclonal antibodies are antibodies that are secreted by different B cell lineages within the host (whereas monoclonal antibodies come from a single cell lineage). They are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Choose between properties like host, clone and antigen to get the antibody you need. Our staff supports you finding the right product via phone, live chat or email.

If you want to use an unconjugated primary, don't forget the corresponding secondary antibody!

Important Targets

beta Actin Antibodies

beta Amyloid Antibodies

Caspase 8 Antibodies

beta Catenin Antibodies

COL1 Antibodies

NFKB1 Antibodies

c-FOS Antibodies

TNF alpha Antibodies

WNT2 Antibodies

All Polyclonal Antibodies

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603,660 results :

Calretinin 抗体

CALB2 适用: 人 WB, IHC (p), IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Calbindin 2 (CALB2) antibody (ABIN2855596)

IHC-P Image Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin, antibody at 1:250 dilution.

WB Image Calretinin antibody detects CALB2 protein by Western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A431 , 12% SDS PAGE Calretinin antibody antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calretinin , antibody at 1:200 dilution.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE antibody diluted at 1:1000

IHC-P Image Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.

7 images

1 reference

Independent Validation

1 validation

产品编号 ABIN2855596

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Datasheet as PDF

beta Amyloid 抗体 (N-Term)

Abeta 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037)

Western Blot of Rabbit anti-Beta Amyloid antibody.

Immunofluorescence microscopy of Rabbit Anti-Beta Amyloid antibody using HeLa cells fixed with MeOH.

Immunohistochemistry with anti-beta amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C).

ELISA results of purified Rabbit anti-Beta Amyloid Antibody tested against BSA-conjugated peptide of immunizing peptide.

Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Western Blot of Rabbit anti-Beta Amyloid Antibody.

Immunofluorescence (IF) image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037)

9 images

1 reference

Independent Validation

2 validations

产品编号 ABIN95037

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Datasheet as PDF

Collagen IV 抗体

COL4 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

anti collagen IV antibody (600-401-106 Lot 25440, 1:400, 45 min RT) showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli; and liver (Right) with strong staining in sinusoids.

SMFC tracking in metamorphosed newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

Lamina lucida and lamina densa regenerate before new ECM deposition.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in metamorphosed newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in metamorphosed newt limb regeneration.

12 images

8 references

Independent Validation

1 validation

产品编号 ABIN5596835

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Datasheet as PDF

COL3 抗体

COL3 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type III (COL3) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III (COL3) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III (COL3) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III (COL3) antibody (ABIN5596830)

anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules.

Western Blot of Rabbit Anti-COLLAGEN III Antibody Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4°C Secondary antibody: 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block: ABIN925618 for 30 min at RT Predicted/Observed size: 138 kDa, 138 kDa

Immunohistochemistry of Rabbit Anti-collagen type III antibody.

(a) Typical electropherogram of α (I) subunits, oligomers β/γ (I), and collagen type I degradation products, released from healthy and burnt skin.

Typical electropherogram of α (III) subunits, oligomers β/γ (III), and collagen type III degradation products, released from healthy and burned skin.

9 images

5 references

Independent Validation

1 validation

产品编号 ABIN5596830

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Datasheet as PDF

Collagen Type I 抗体

COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated

Decreases in the levels of activated HSC markers, SOX9, COL1 and phosphoMyl9 (PMYL9) following PAK-1 abrogation by siRNA1 in activated rat HSCs relative to their respective scrambled control levels.

Characterisation of COL1 expression in control and Sox9-null animals following liver fibrosis induction using ABIN135046.

Activated mouse HSCs (‘Control') show decreased protein levels for α-SMA, COL1 and SOX9 by immunoblotting following the loss of integrin beta-1 (‘Itgb1-null').

Elevated collagen levels in skeletal muscles in SGCA- and SGCD-null mice and in the heart of SGCD-null mice investigated using ABIN135046: Immunofluorescent images of skeletal muscles stained with collagen type I (fibrotic marker, green) and laminin (extracellular matrix of muscle fibres, red) Scale bar: 100 μm.

Immunofluorescence images of the heart stained with collagen type I (fibrotic marker, green) using ABIN135046.

(A) Effects on collagen deposition in indirect (inserts) and direct coculture of human dermal fibroblasts and siRNA-treated THP1 cells.

Immunoblot analysis of primary AF and NP cell lysates from two independent donors, D1 (P0) and D3 (P1), respectively, for COL1A1 (ABIN135046) and COL2A1 (ABIN135048).

Immunoblot Analysis using ABIN135046.

8 images

69 references

产品编号 ABIN135046

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Datasheet as PDF

Histone 3 抗体 (H3K27ac)

H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K27ac) antibody (ABIN2668475)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K27ac) antibody (ABIN2668475)

Histone H3 acetyl Lys27 antibody tested by Western blot.

Histone H3 acetyl Lys27 antibody (pAb) tested by ChIP-chip.

Histone H3 acetyl Lys27 antibody tested by dot blot analysis.

Histone H3 acetyl Lys27 antibody tested by ChIP qPCR analysis.

Histone H3 acetyl Lys27 antibody tested by immunofluorescence.

7 images

23 references

Independent Validation

1 validation

产品编号 ABIN2668475

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Datasheet as PDF

GLI2 抗体 (Middle Region)

GLI2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody (ABIN2777474)

Immunohistochemistry validation image for anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody (ABIN2777474)

In Fig.

Host: Rabbit Target Name: GLI2 Sample Type: Jurkat Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.

WB Suggested Anti-GLI2 Antibody Titration: 1.

Sample Type : Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop Primary Antibody Dilution : 1:50 Secondary Antibody : Goat anti-rabbit-cy3 Secondary Antibody Dilution : 1:1500 Color/Signal Descriptions : GLI2: Green Neural crest cells:: Red Gene Name : GLI2 Submitted by : Anonymous

Host: Rabbit Target Name: GLI2 Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1.

Rabbit Anti-GLI2 Antibody Paraffin Embedded Tissue: Human Heart Cellular Data: Myocardial cells Antibody Concentration: 4.

Rabbit Anti-GLI2 Antibody Paraffin Embedded Tissue: Human Intestine Cellular Data: Epithelial cells of intestinal gland Antibody Concentration: 4.

9 images

23 references

Independent Validation

2 validations

产品编号 ABIN2777474

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Datasheet as PDF

PI3K p85 alpha/gamma 抗体 (pTyr199, pTyr467)

PI3K p85a/g 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g) (pTyr199), (pTyr467) antibody (ABIN744743)

Image provided by Independent Validation Program, badge 29801.

Formalin-fixed and paraffin embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin-embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining

5 images

3 references

Independent Validation

1 validation

产品编号 ABIN744743

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Datasheet as PDF

PD-1 抗体 (AA 201-288)

PDCD1 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Programmed Cell Death 1 (PDCD1) (AA 201-288) antibody (ABIN735608)

Immunohistochemistry validation image for anti-Programmed Cell Death 1 (PDCD1) (AA 201-288) antibody (ABIN735608)

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-D-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-PD-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

L1 human kidney lysates L2 rat brain lysates probed with Anti PD-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 overnight at 4 °C.

5 images

2 references

Independent Validation

1 validation

产品编号 ABIN735608

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Datasheet as PDF

AKT 1/2/3 抗体 (AA 401-479)

适用: 人, 小鼠, 大鼠, Cow, Pig WB, IF (cc), IF (p), FACS, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Flow Cytometry validation image for anti-AKT 1/2/3 (AA 401-479) antibody (ABIN725195)

Flow Cytometry validation image for anti-AKT 1/2/3 (AA 401-479) antibody (ABIN725195)

Mouse placenta lysate with Anti-AKT1/3 Polyclonal Antibody Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: Mouse Placenta lysates；Lane 2: Mouse Lung lysates probed with AKT1/3 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

5 images

9 references

Independent Validation

1 validation

产品编号 ABIN725195

获取报价

Datasheet as PDF

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