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Polyclonal Antibodies

Polyclonal antibodies are antibodies that are secreted by different B cell lineages within the host (whereas monoclonal antibodies come from a single cell lineage). They are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Choose between properties like host, clone and antigen to get the antibody you need. Our staff supports you finding the right product via phone, live chat or email.

If you want to use an unconjugated primary, don't forget the corresponding secondary antibody!

Important Targets

beta Actin Antibodies

beta Amyloid Antibodies

Caspase 8 Antibodies

beta Catenin Antibodies

COL1 Antibodies

NFKB1 Antibodies

c-FOS Antibodies

TNF alpha Antibodies

WNT2 Antibodies

All Polyclonal Antibodies

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568,073 results :

LEF1 抗体 (AA 100-399)

LEF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 100-399) antibody (ABIN1680678)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 100-399) antibody (ABIN1680678)

Western blot analysis of extracts of various cell lines, using LEF1 antibody (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at 1:1000 dilution.

Immunofluorescence analysis of rat thymus cells using LEF1 Rabbit pAb (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at dilution of 1:25 (40x lens).

Alignment tracks from CUT&RUN targeting LEF1 in HEK293T cells using anti-LEF1 antibody ABIN1680678 (1).

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg LEF1 antibody (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021).

Immunofluorescence analysis of mouse thymus cells using LEF1 Rabbit pAb (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at dilution of 1:25 (40x lens).

8 images

1 reference

Independent Validation

1 validation

产品编号 ABIN1680678

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Datasheet as PDF

TCF7L1 抗体 (N-Term)

TCF7L1 适用: 人 WB, ChIP-seq, ChIP, CUT&RUN 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1) (N-Term) antibody (ABIN6972849)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1) (N-Term) antibody (ABIN6972849)

Western blot of TCF7L1 / TCF3 antibody.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

TCF7L1 / TCF3 antibody (pAb) tested by ChIP-Seq.

Alignment tracks from CUT&RUN targeting TCF7L1 in HEK293T cells using anti-TCF7L1 antibody ABIN6972849 (1).

6 images

1 reference

Independent Validation

1 validation

产品编号 ABIN6972849

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Datasheet as PDF

NFKB1 抗体 (AA 740-964)

NFKB1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 740-964) antibody (ABIN6144571)

Western blot analysis of extracts of various cell lines, using NFKB1 antibody.

Immunofluorescence analysis of U2OS cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using NFKB1 antibody.

Immunofluorescence analysis of A549 cells using NFKB1 antibody.

Immunofluorescence analysis of A-549 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunofluorescence analysis of NIH/3T3 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

8 images

2 references

Independent Validation

1 validation

产品编号 ABIN6144571

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Datasheet as PDF

Complement Factor H 抗体 (AA 20-270)

CFH 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Complement Factor H (CFH) (AA 20-270) antibody (ABIN3023097)

Western blot analysis of extracts of HepG2 cell line, using CFH antibody.

Immunohistochemistry of paraffin-embedded human placenta using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of Rat liver, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of Human plasma, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of various cell lines, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human liver cancer using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HepG2 cells using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

8 images

1 reference

Independent Validation

1 validation

产品编号 ABIN3023097

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Datasheet as PDF

Histone 3 抗体 (H3K4me)

H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded human kidney cancer tissue using H3K4me2 antibody at dilution of 1:200 (x400 lens).

Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat brain using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Rat kidney using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K4 antibody.

Dot Blot (DB) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Mouse brain using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat liver using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat liver using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K4 antibody.

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry (IHC) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K4 antibody.

Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry (IHC) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded human kidney cancer using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Mouse lung using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Mouse brain using DiMethyl-Histone H3-K4 antibody.

23 images

4 references

Independent Validation

1 validation

产品编号 ABIN3023251

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Datasheet as PDF

Calretinin 抗体

CALB2 适用: 人 WB, IHC (p), IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Calbindin 2 (CALB2) antibody (ABIN2855596)

IHC-P Image Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin, antibody at 1:250 dilution.

WB Image Calretinin antibody detects CALB2 protein by Western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A431 , 12% SDS PAGE Calretinin antibody antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calretinin , antibody at 1:200 dilution.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE antibody diluted at 1:1000

IHC-P Image Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.

7 images

1 reference

Independent Validation

1 validation

产品编号 ABIN2855596

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Datasheet as PDF

CTNNB1 抗体 (N-Term)

KO Validated CTNNB1 适用: 人 WB, IHC, IF, IHC (p), FACS, IP, ICC, IHC (fro), ChIP, CUT&RUN, IHC (wm) 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

WB Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: HeLa 7.

IHC-P Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin, antibody (Green) at 1:200 dilution.

WB Image beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by Western blot analysis.

IP Image Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody, Western blot analysis was performed using beta Catenin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Catenin, antibody at 1:500 dilution.

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells.

Expression analysis of Wnt pathway genes in human OS cellsWestern blot analysis of active β-catenin, total β-catenin and β-actin proteins in human OS cell lines (143B, Saos-2, SJSA-1 , U-2 OS, MG-63) with human breast cancer cell high-level-expression controls (MCF7, MDA-MB-231) and additional human mesenchymal stem cell low-level-expression controls (hMSC).

SGSM2 silencing affected T47D cell adhesion and cell migration.

Interaction between SGSM2 and E-cadherin was verified by co-immunoprecipitation and immunofluorescence staining assays.

16 images

8 references

Independent Validation

1 validation

产品编号 ABIN2855042

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Datasheet as PDF

HDAC1 抗体

KO Validated HDAC1 适用: 人 WB, IF, IP, IHC (p), ICC, ChIP, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone Deacetylase 1 (HDAC1) antibody (ABIN2854776)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone Deacetylase 1 (HDAC1) antibody (ABIN2854776)

WB Image HDAC1 antibody detects HDAC1 protein by western blot analysis.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

IHC-P Image Immunohistochemical analysis of paraffin-embedded human testis, using HDAC1, antibody(10 μg/ml).

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus on mouse colon by immunohistochemical analysis.

ICC/IF Image HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.

IHC-P Image HDAC1 antibody detects HDAC1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.

ChIP Image HDAC1 antibody immunoprecipitates HDAC1 protein-DNA in ChIP experiments.

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus in human lung adenocarcinoma by immunohistochemical analysis.

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus on mouse liver by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using HDAC1, antibody at 1:200 dilution.

IP Image HDAC1antibody immunoprecipitates HDAC1 protein in IP experiments.

HDAC1/2 depletion in adult Schwann cells leads to motor and sensory dysfunction in peripheral nerves.

HDAC1/2 depletion in adult Schwann cells leads to motor and sensory dysfunction in peripheral nerves.

SAHA affects the expression of suvivin and XIAP at the transcriptional level.

HDAC1/2 are robustly upregulated in SCs after lesion.

HDAC1/2 are robustly upregulated in SCs after lesion.

Overexpression of peptidase inhibitor 16 (PI16) attenuates angiotensin II (Ang II)-induced fibrotic gene expression and histone 3 modification in NRCFs.

The effects of peptidase inhibitor 16 (PI16) on newborn rat cardiac fibroblasts (NRCFs) are partially rescued by overexpression of HDAC1.

The effects of peptidase inhibitor 16 (PI16) on newborn rat cardiac fibroblasts (NRCFs) are partially rescued by overexpression of HDAC1.

Alignment tracks from CUT&RUN targeting HDAC1 in Mouse fore limb (11.

22 images

7 references

Independent Validation

1 validation

产品编号 ABIN2854776

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Datasheet as PDF

STAT3 抗体 (C-Term)

KO Validated STAT3 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865)

WB Image Sample (20 ug of whole cell lysate) A: human ESC 7.

WB Image Sample(30 ug whole cell lysate) A: 293T B: A431 , C: H1299 D: HeLa S3 , E: Hep G2 , F: MOLT4 , 7.

WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis.

WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT3, antibody at 1:200 dilution.

Effects of treatment with antrodan only, cisplatin only, and a combination of both on protein expression of MMP-2 and MMP-9, and on the phosphorylation and protein expression of signal transducer and activator of transcription 3 (STAT3), extracellular signal-regulated kinase 1 (ERK-1), c-Jun N-terminal kinase 1 (JNK1), JNK2, and p38 in lung tissues of C57BL/6 mice.

Effects of treatment with antrodan only, cisplatin only, and a combination of both on protein expression of MMP-2 and MMP-9, and on the phosphorylation and protein expression of STAT3, ERK1, JNK1, JNK2, and p38 in liver tissues of C57BL/6 mice.

MLN8237 treatment resulted in abnormal activation of Akt/Stat3 pathway.

10 images

1 reference

Independent Validation

1 validation

产品编号 ABIN2855865

获取报价

Datasheet as PDF

CBP 抗体 (C-Term)

KO Validated CREBBP 适用: 人 WB, IF, IP, IHC (p), ICC, ChIP 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-CREB Binding Protein (CREBBP) (C-Term) antibody (ABIN2854987)

Cleavage Under Targets and Release Using Nuclease validation image for anti-CREB Binding Protein (CREBBP) (C-Term) antibody (ABIN2854987)

WB Image Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE antibody diluted at 1:1000

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse skin by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on human breast carcinoma by immunohistochemical analysis.

IP Image CBP antibody immunoprecipitates CBP protein in IP experiments.

ChIP Image CREBBP antibody immunoprecipitates CREBBP protein-DNA complex in ChIP experiments.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on human cervical carcinoma by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse muscle by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse fore brain by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBP , antibody at 1:200 dilution.

11 images

1 reference

Independent Validation

1 validation

产品编号 ABIN2854987

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Datasheet as PDF

Collagen Type I 抗体

COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated

Decreases in the levels of activated HSC markers, SOX9, COL1 and phosphoMyl9 (PMYL9) following PAK-1 abrogation by siRNA1 in activated rat HSCs relative to their respective scrambled control levels.

Characterisation of COL1 expression in control and Sox9-null animals following liver fibrosis induction using ABIN135046.

Activated mouse HSCs (‘Control') show decreased protein levels for α-SMA, COL1 and SOX9 by immunoblotting following the loss of integrin beta-1 (‘Itgb1-null').

Elevated collagen levels in skeletal muscles in SGCA- and SGCD-null mice and in the heart of SGCD-null mice investigated using ABIN135046: Immunofluorescent images of skeletal muscles stained with collagen type I (fibrotic marker, green) and laminin (extracellular matrix of muscle fibres, red) Scale bar: 100 μm.

Immunofluorescence images of the heart stained with collagen type I (fibrotic marker, green) using ABIN135046.

(A) Effects on collagen deposition in indirect (inserts) and direct coculture of human dermal fibroblasts and siRNA-treated THP1 cells.

Immunoblot analysis of primary AF and NP cell lysates from two independent donors, D1 (P0) and D3 (P1), respectively, for COL1A1 (ABIN135046) and COL2A1 (ABIN135048).

Immunoblot Analysis using ABIN135046.

8 images

69 references

产品编号 ABIN135046

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Datasheet as PDF

Histone 3 抗体 (H3K27ac)

H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K27ac) antibody (ABIN2668475)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K27ac) antibody (ABIN2668475)

Histone H3 acetyl Lys27 antibody tested by Western blot.

Histone H3 acetyl Lys27 antibody (pAb) tested by ChIP-chip.

Histone H3 acetyl Lys27 antibody tested by dot blot analysis.

Histone H3 acetyl Lys27 antibody tested by ChIP qPCR analysis.

Histone H3 acetyl Lys27 antibody tested by immunofluorescence.

7 images

23 references

Independent Validation

1 validation

产品编号 ABIN2668475

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Datasheet as PDF

GLI2 抗体 (Middle Region)

GLI2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody (ABIN2777474)

Immunohistochemistry validation image for anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody (ABIN2777474)

In Fig.

Host: Rabbit Target Name: GLI2 Sample Type: Jurkat Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.

WB Suggested Anti-GLI2 Antibody Titration: 1.

Sample Type : Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop Primary Antibody Dilution : 1:50 Secondary Antibody : Goat anti-rabbit-cy3 Secondary Antibody Dilution : 1:1500 Color/Signal Descriptions : GLI2: Green Neural crest cells:: Red Gene Name : GLI2 Submitted by : Anonymous

Host: Rabbit Target Name: GLI2 Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1.

Rabbit Anti-GLI2 Antibody Paraffin Embedded Tissue: Human Heart Cellular Data: Myocardial cells Antibody Concentration: 4.

Rabbit Anti-GLI2 Antibody Paraffin Embedded Tissue: Human Intestine Cellular Data: Epithelial cells of intestinal gland Antibody Concentration: 4.

9 images

23 references

Independent Validation

2 validations

产品编号 ABIN2777474

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Datasheet as PDF

DYKDDDDK Tag 抗体

Rockland 600-401-383 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

Immunofluorescence validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

Affinity Purified Antibody to detect conjugated proteins detects both C terminal linked and N terminal linked tagged recombinant proteins by western blot.

Western Blot of Rabbit anti-FLAG antibody.

antibody to detect conjugated proteins is shown to detect as little as 3 ng of amino-terminal tagged recombinant protein by western blot.

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

8 images

20 references

Independent Validation

2 validations

产品编号 ABIN99294

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Datasheet as PDF

PI3K p85 alpha/gamma 抗体 (pTyr199, pTyr467)

PI3K p85a/g 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g) (pTyr199), (pTyr467) antibody (ABIN744743)

Image provided by Independent Validation Program, badge 29801.

Formalin-fixed and paraffin embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin-embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining

5 images

3 references

Independent Validation

1 validation

产品编号 ABIN744743

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Datasheet as PDF

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