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Glycerol Kinase 抗体 (Biotin)

Rockland 200-106-211-0100 GK 适用: Cellulomonas ELISA, WB 宿主: 山羊 Polyclonal Biotin
Rockland
产品编号 ABIN99893
Supplier Product No.: 200-106-211-0100
发货至: 中国
  • 抗原 See all Glycerol Kinase (GK) 抗体
    Glycerol Kinase (GK)
    适用
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    Cellulomonas
    宿主
    • 75
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    山羊
    克隆类型
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    多克隆
    标记
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    This Glycerol Kinase antibody is conjugated to Biotin
    应用范围
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    ELISA, Western Blotting (WB)
    Supplier Product No.
    200-106-211-0100
    Supplier
    Rockland
    原理
    Glycerol Kinase Antibody Biotin Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glycerol Kinase [Cellulomonas species].
    产品特性
    Synonyms: goat anti-Glycerol Kinase Antibody biotin Conjugation, biotin Conjugated goat anti-Glycerol Kinase Antibody, ATP glycerol 3 phosphotransferase antibody, GK antibody, GK antibody, GK1 antibody, GKD antibody, Glycerokinase antibody, Glycerol kinase antibody, Glycerol kinase deficiency antibody
    纯化方法
    Anti-Glycerol Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Glycerol Kinase [Cellulomonas species]

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product GK Primary Antibody
  • 应用备注

    Application Note: Anti-Glycerol Kinase has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Glycerol Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:2,500

    ELISA Dilution: 1:1,000 - 1:5,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    10.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • 抗原
    Glycerol Kinase (GK)
    别名
    glpK (GK 产品)
    背景
    Background: Anti-Glycerol Kinase antibody recognizes the phosphotransferase enzyme glycerol kinase. Glycerol kinase catalyzes the formation of glycerol phosphate by transferring a phosphate from ATP to glycerol. Dihydroxyacetone and L-glyceraldehyde can also act as acceptors in place of glycerol. Anti-Glycerol Kinase is ideal for investigators interested in Cancer, Metabolism, and Kinase/Phosphatase enzymology.
    基因ID
    6960048
    UniProt
    D0VZG4
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