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GST 抗体 (Biotin)

Rockland 600-106-200 GST 适用: Schistosoma japonicum WB, ELISA, IHC 宿主: 山羊 Polyclonal Biotin
Rockland
产品编号 ABIN99864
Supplier Product No.: 600-106-200
发货至: 中国
  • 抗原 See all GST 抗体
    GST (Glutathione S Transferase (GST))
    适用
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    Schistosoma japonicum
    宿主
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    山羊
    克隆类型
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    多克隆
    标记
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    This GST antibody is conjugated to Biotin
    应用范围
    • 141
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-106-200
    Supplier
    Rockland
    原理
    GST Antibody Biotin Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
    产品特性
    Synonyms: goat anti-GST antibody biotin conjugation, biotin conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody
    纯化方法
    Anti-GST Antibody, Biotin Conjugated, was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product GST Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-GST Antibody, Biotin Conjugated has been tested by ELISA and is suitable for immunoblotting, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.

    Western Blot Dilution: 1:2,000 - 1:10,000

    ELISA Dilution: 1:10,000 - 1:50,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • Helleboid, Haug, Lamottke, Zhou, Wei, Daix, Cambula, Rigou, Hum, Walczak: "The Identification of Naturally Occurring Neoruscogenin as a Bioavailable, Potent, and High-Affinity Agonist of the Nuclear Receptor RORα (NR1F1)." in: Journal of biomolecular screening, (2013) (PubMed).

  • 抗原
    GST (Glutathione S Transferase (GST))
    别名
    GST (GST 产品)
    背景
    Background: Biotin conjugated anti-GST antibody is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.
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