GST 抗体
(7 references)
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- 抗原 See all GST 抗体
- GST (Glutathione S Transferase (GST))
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适用
- Schistosoma japonicum
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GST antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
- Supplier Product No.
- 600-101-200
- Supplier
- Rockland
- 原理
- GST Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
- 产品特性
- Synonyms: goat anti-GST antibody, Glutathione-S-Transferase
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product GST Primary Antibody
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anti-Glutathione S Transferase (GST) antibody
GST 适用: Schistosoma japonicum WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibody (Biotin)GST 适用: 化学剂 WB, ELISA 宿主: 小鼠 Monoclonal vpg66 Biotin
anti-Glutathione S Transferase (GST) antibodyGST 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1-00E-05 unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP 宿主: 小鼠 Monoclonal 2F10B9 unconjugated
anti-Glutathione S Transferase (GST) (AA 1-218) antibodyGST 适用: Various Species WB, IP, DB 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) (AA 1-218) antibodyGST 适用: Various Species WB, IP, DB 宿主: 小鼠 Monoclonal unconjugated
anti-Glutathione S Transferase (GST) antibody (Alkaline Phosphatase (AP))GST 适用: Schistosoma japonicum WB, ELISA 宿主: 小鼠 Monoclonal 3E2 Alkaline Phosphatase (AP)
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:10,000
Application Note: Anti-GST has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This antibody has also been reported to be suitable for immobilization in Label-free Interaction Analysis (Biocore).
Western Blot Dilution: 1:1,000 - 1:10,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:20,000 - 1:50,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store GST antibody at 4° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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: "Inosine induces context-dependent recoding and translational stalling." in: Nucleic acids research, Vol. 47, Issue 1, pp. 3-14, (2019) (PubMed).
: "Identification of stroke-associated-antigens via screening of recombinant proteins from the human expression cDNA library (SEREX)." in: Journal of translational medicine, Vol. 13, pp. 71, (2016) (PubMed).
: "Analysis of site-specific N-glycan remodeling in the endoplasmic reticulum and the Golgi." in: Glycobiology, Vol. 25, Issue 12, pp. 1335-49, (2016) (PubMed).
: "Cdc42p-interacting protein Bem4p regulates the filamentous-growth mitogen-activated protein kinase pathway." in: Molecular and cellular biology, Vol. 35, Issue 2, pp. 417-36, (2015) (PubMed).
: "The filamentous growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 transcriptional repressors in Saccharomyces cerevisiae." in: Genetics, Vol. 192, Issue 3, pp. 869-87, (2013) (PubMed).
: "Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1." in: Cell death and differentiation, Vol. 19, Issue 7, pp. 1152-61, (2012) (PubMed).
: "Using affinity chromatography to engineer and characterize pH-dependent protein switches." in: Protein science : a publication of the Protein Society, Vol. 18, Issue 1, pp. 217-28, (2009) (PubMed).
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: "Inosine induces context-dependent recoding and translational stalling." in: Nucleic acids research, Vol. 47, Issue 1, pp. 3-14, (2019) (PubMed).
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- 抗原
- GST (Glutathione S Transferase (GST))
- 别名
- GST (GST 产品)
- 背景
- Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
- UniProt
- P08515
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