GLN1 抗体
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- 抗原 See all GLN1 抗体
- GLN1 (Glutamine Synthetase (GLN1))
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适用
- Brevibacterium
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GLN1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), ELISA
- 原理
- Glutamine Synthetase Antibody
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glutamine Synthetase [Microbial].
- 产品特性
- Synonyms: Glutamine synthetase, goat anti-Glutamine Synthetase Antibody
- 纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- 免疫原
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Immunogen: Glutamine Synthetase [native from brevibacterium species]
Immunogen Type: Native Protein
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- Discover our top product GLN1 Primary Antibody
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- 应用备注
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Application Note: Anti-Glutamine Synthetase has been tested by western blot and is suitable to be assayed against 1.0 μg of Glutamine Synthetase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 90 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- GLN1 (Glutamine Synthetase (GLN1))
- 别名
- Glutamine Synthetase (GLN1 产品)
- 背景
- Background: Catalytic activity of Glutamine Synthetase is ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
- 基因ID
- 3345165
- UniProt
- Q79VE3
- 途径
- Positive Regulation of Peptide Hormone Secretion
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