(8 references)-
- 抗原 See all GLUD1 抗体
- GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GLUD1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 原理
- Glutamate Dehydrogenase Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Glutamate Dehydrogenase [Bovine Liver].
- 产品特性
- Synonyms: rabbit anti-Glutamate Dehydrogenase Antibody, Glutamate dehydrogenase 1 mitochondrial, GDH 1
- 纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- 免疫原
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Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
Immunogen Type: Native Protein
- Top Product
- Discover our top product GLUD1 Primary Antibody
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anti-Glutamate Dehydrogenase 1 (GLUD1) antibody
GLUD1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 54-240) antibodyGLUD1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) antibodyGLUD1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 犬, 兔, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 65-273) antibodyGLUD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 犬, 绵羊, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 182-231) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 兔, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) antibodyGLUD1 适用: 人 WB, ELISA, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 93-107) antibodyGLUD1 适用: 人, 小鼠, 马, Pig, 猴 WB, IHC, ELISA, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: This antibody has been tested by western blot. Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect a band approximately 56 kDa in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract. Anti-Glutamate Dehydrogenase Antibody is suitable for use in ELISA.
Western Blot Dilution: 1:1,000 - 1:3,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:4,000 - 1:16,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 85 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid oxidation." in: Human molecular genetics, Vol. 23, Issue 13, pp. 3513-22, (2015) (PubMed).
: "Deletion of Glud1 (Glutamate Dehydrogenase 1) in the Central Nervous System affects glutamate handling without altering synaptic transmission." in: Journal of neurochemistry, (2012) (PubMed).
: "Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6." in: BMC cell biology, Vol. 10, pp. 58, (2009) (PubMed).
: "Identification of a novel PEX14 mutation in Zellweger syndrome." in: Journal of medical genetics, Vol. 45, Issue 6, pp. 376-83, (2008) (PubMed).
: "Oxidative modification of proteins in the frontal cortex of Alzheimer's disease brain." in: Neurobiology of aging, Vol. 27, Issue 1, pp. 42-53, (2005) (PubMed).
: "Insulin secretion profiles are modified by overexpression of glutamate dehydrogenase in pancreatic islets." in: Diabetologia, Vol. 47, Issue 2, pp. 266-76, (2004) (PubMed).
: "Potential role for Pex19p in assembly of PTS-receptor docking complexes." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12615-24, (2004) (PubMed).
: "Biochemical and immunohistochemical studies on tropomyosin and glutamate dehydrogenase in the chicken liver." in: Zoological science, Vol. 19, Issue 3, pp. 275-86, (2002) (PubMed).
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: "Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid oxidation." in: Human molecular genetics, Vol. 23, Issue 13, pp. 3513-22, (2015) (PubMed).
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- 抗原
- GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))
- 别名
- GLUD1 (GLUD1 产品)
- 背景
- Background: Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1, EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.
- 基因ID
- 281785, 32880221
- UniProt
- P00366
- 途径
- Positive Regulation of Peptide Hormone Secretion, Warburg Effect
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