Glucose Oxidase 抗体
Quick Overview for Glucose Oxidase 抗体 (ABIN99703)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-1137
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Supplier
- Rockland
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原理
- Glucose Oxidase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glucose Oxidase [Aspergillus niger].
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产品特性
- Synonyms: goat anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
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纯化方法
- Anti-glucose oxidase antibody was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
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Immunogen: Glucose Oxidase [Aspergillus niger]
Immunogen Type: Native Protein
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anti-Glucose Oxidase (GOD) antibody
GOD 适用: Aspergillus niger ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Application Note: Anti-glucose oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Optimal concentration should be determined by the researcher.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 90 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Glucose Oxidase (GOD)
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别名
- Glucose Oxidase
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背景
- Background: Glucose oxidase antibody is specific to glucose oxidase enzyme. Glucose oxidase is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. Glucose oxidase is commonly used for the determination of free glucose in body fluids (diagnostics), and in the food industry. It also has many applications in biotechnologies, for example enzyme assays for biochemistry including biosensors in nanotechnologies. Glucose oxidase is often extracted from Aspergillus niger.
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UniProt
- P13006
抗原
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