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Fructosyl Amino Acid Oxidase 抗体

Rockland 200-601-236-0100 socD 适用: Corynebacterium WB, IP, ELISA 宿主: 绵羊 Polyclonal unconjugated
Rockland
产品编号 ABIN99448
Supplier Product No.: 200-601-236-0100
发货至: 中国
  • 抗原 See all Fructosyl Amino Acid Oxidase (socD) products
    Fructosyl Amino Acid Oxidase (socD)
    适用
    • 2
    • 1
    • 1
    Corynebacterium
    宿主
    • 3
    • 1
    绵羊
    克隆类型
    • 4
    多克隆
    标记
    • 3
    • 1
    This Fructosyl Amino Acid Oxidase antibody is un-conjugated
    应用范围
    • 4
    • 4
    • 2
    • 2
    • 2
    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    Supplier Product No.
    200-601-236-0100
    Supplier
    Rockland
    原理
    Fructosyl-Amino Acid Oxidase Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase [E.
    产品特性
    Synonyms: sheep anti-Fructosyl-Amino Acid Oxidase Antibody, Fructosyl-amino acid oxidase
    纯化方法
    Anti-FRUCTOSYL-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]

    Immunogen Type: Recombinant Protein

    亚型
    IgG
  • 应用备注

    Application Note: Fructosyl-amino acid oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Fructosyl-Amino Acid Oxidase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • 抗原
    Fructosyl Amino Acid Oxidase (socD)
    别名
    Fructosyl-Amino Acid Oxidase (socD 产品)
    背景
    Background: Fructosyl amino acid oxidase is a flavoprotein that catalyzes the oxidation of fructosyl amino acids to form glucosone, amino acid and hydrogen peroxide. Anti-Fructosyl-amino acid oxidase can be useful for researchers interested in diabetes research.
    UniProt
    Q8RIU8
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