PFKM 抗体
(1 reference)
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- 抗原 See all PFKM 抗体
- PFKM (phosphofructokinase, Muscle (PFKM))
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PFKM antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 100-1156
- Supplier
- Rockland
- 原理
- Fructose-6-Phosphate Kinase Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle].
- 产品特性
- Synonyms: goat anti-Fructose-6-Phosphate Kinase Antibody, 6 Phosphofructokinase Muscle Type antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody
- 纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- 免疫原
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Immunogen: Fructose-6-Phosphate Kinase [Rabbit Muscle]
Immunogen Type: Native Protein
- Top Product
- Discover our top product PFKM Primary Antibody
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anti-phosphofructokinase, Muscle (PFKM) (AA 2-390) antibody
PFKM 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 501-780) antibodyPFKM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 166-334) antibodyPFKM 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) antibodyPFKM 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 1-780) antibodyPFKM 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 681-780) antibodyPFKM 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D1 unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 746-776), (C-Term) antibodyPFKM 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB3908 unconjugated
anti-phosphofructokinase, Muscle (PFKM) antibodyPFKM 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 1-780) antibodyPFKM 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (N-Term) antibodyKO Validated PFKM 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: This antibody suitable for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 90 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Molecular complex of three testis-specific isozymes associated with the mouse sperm fibrous sheath: hexokinase 1, phosphofructokinase M, and glutathione S-transferase mu class 5." in: Biology of reproduction, Vol. 82, Issue 3, pp. 504-15, (2010) (PubMed).
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: "Molecular complex of three testis-specific isozymes associated with the mouse sperm fibrous sheath: hexokinase 1, phosphofructokinase M, and glutathione S-transferase mu class 5." in: Biology of reproduction, Vol. 82, Issue 3, pp. 504-15, (2010) (PubMed).
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- 抗原
- PFKM (phosphofructokinase, Muscle (PFKM))
- 别名
- PFKM (PFKM 产品)
- 背景
- Background: Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation. This is a unidirectional and rate-limiting step in glycolysis. Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate. The enzyme exists as a homotetramer.
- 基因ID
- 100345647, 125128
- UniProt
- P00511
- 途径
- Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect
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