Fluorescein 抗体
(9 references)
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- 抗原
- Fluorescein
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
- Supplier Product No.
- 600-101-096
- Supplier
- Rockland
- 原理
- Fluorescein (FITC) Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.
- 产品特性
- Synonyms: goat Anti FITC, goat anti Fluorescein
- 纯化方法
- Fluorescein Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein conjugated Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Fluorescein conjugated to Goat IgG
Immunogen Type: Other
- 亚型
- IgG
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anti-Fluorescein antibody
WB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
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- 应用备注
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 1:250 - 1:1,500
Application Note: Anti-Fluorescein Antibody has been tested by ELISA and western blot and is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems." in: PLoS ONE, Vol. 15, Issue 4, pp. e0232046, (2020) (PubMed).
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: "Bach1 siRNA attenuates bleomycin-induced pulmonary fibrosis by modulating oxidative stress in mice." in: International journal of molecular medicine, Vol. 39, Issue 1, pp. 91-100, (2017) (PubMed).
: "Activation of cannabinoid receptor type 2 by JWH133 alleviates bleomycin-induced pulmonary fibrosis in mice." in: Oncotarget, Vol. 8, Issue 61, pp. 103486-103498, (2017) (PubMed).
: "Survival of irradiated recipient mice after transplantation of bone marrow from young, old and "early aging" mice." in: Aging, Vol. 7, Issue 12, pp. 1212-23, (2016) (PubMed).
: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).
: "Direct and indirect contribution of bone marrow-derived cells to cancer." in: International journal of cancer, Vol. 126, Issue 10, pp. 2308-18, (2010) (PubMed).
: "Fluorescein: a rapid, sensitive, nonlethal method for detecting skin ulceration in fish." in: Veterinary pathology, Vol. 39, Issue 6, pp. 726-31, (2002) (PubMed).
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: "Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems." in: PLoS ONE, Vol. 15, Issue 4, pp. e0232046, (2020) (PubMed).
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- 抗原
- Fluorescein
- 物质类
- Chemical
- 背景
- Background: Anti-Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti-FITC is also suitable as a visualization reagent for fluorescein-labelled compounds (such as antibodies, lectins and peptide nucleic acid (PNA) probes).
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