Fetuin A 抗体
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- 抗原 See all Fetuin A (AHSG) 抗体
- Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Fetuin A antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-101-456
- Supplier
- Rockland
- 原理
- Fetuin Antibody
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.
- 产品特性
- Synonyms: goat anti-Fetuin antibody, 59 kDa bone sialic acid-containing protein antibody, A2HS antibody, Aa2-066 antibody, AHS antibody, Ahsg alpha-2-HS-glycoprotein antibody, AHSG antibody, Alpha 2 Z globulin antibody, Alpha-2-HS-glycoprotein antibody, Ba-alpha-2-glycoprotein, Fetuin-A, PRO2743
- 纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with a recombinant human fetuin (a2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product AHSG Primary Antibody
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- 应用备注
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Application Note: This purified polyclonal antibody has been tested by western blot. Although not tested, this antibody is likely functional in ELISA, immunohistochemistry, and immunoprecipitation. A doublet band or slightly lower molecular weight band (see image) may be visible by western blot due to proteolytic processing, variable glycosylation and/or phosphorylation. Proteolytic processing may include the removal of a 40 amino acid residue bridging peptide from the A and B chains of fetuin in vivo.
Western Blot Dilution: 1:10,000 - 1:20,000
ELISA Dilution: 1:40.000 - 1:200,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))
- 别名
- AHSG (AHSG 产品)
- 背景
- Background: Human fetuin (2-Heremans-Schmid-glycoprotein or α2-HS-glycoprotein) is a major plasma glycoprotein predominantly synthesized in the liver. Human fetuin is named after its bovine homolog. Fetuins are found in most mammals. Human fetuin is a negative acute-phase protein, normal circulating levels in adults (300-600 μg/mL) fall significantly (30-50 % ) during injury and infection. The biological role of fetuin is unknown, although it has been implicated as an immunomodulator that can participate in stimulation of bacterial phagocytosis by neutrophils and promotion of endocytosis by mouse macrophages. Hepatocytes are the principal cell source of circulating fetuin, but it also is expressed by monocyte/macrophages. Fetuins occur in large amounts in blood and cerebrospinal fluid and accumulate to high concentrations in calcified bone. The fetuin promoter region has several potential interleukin 6-responsive elements, and its synthesis is down-regulated during injury and inflammation. Fetuin is an acidic glycoprotein with three N-linked and three O-linked oligosaccharide chains, whose terminal sugar residues are rich in sialic acid (N-acetylneuraminic acid), contributing to its net negative charge. A role for fetuin as a carrier of bioactive molecules has been proposed based on observations that it binds and carries Ca2+ ion. Fetuin is implicated in bone remodeling, immune function and may play a role in tumor progression of certain cell types.
- 基因ID
- 197
- NCBI登录号
- NP_001613
- UniProt
- P02765
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