(6 references)-
- 抗原 See all Ferritin (FE) 抗体
- Ferritin (FE)
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Ferritin antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 交叉反应 （详细）
- Cross reactivity against Ferritin from other tissues and species may occur but have not been specifically determined.
- 纯度
- Anti-FERRITIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Ferritin [Human Spleen]
Immunogen Type: Native Protein - 亚型
- IgG
- Top Product
- Discover our top product FE Primary Antibody
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anti-Ferritin (FE) antibody
FE 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ferritin (FE) antibodyFE 适用: 人 ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA Dilution: 1:5.000 - 1:20.000
Immunohistochemistry Dilution: 1:200 - 1:1.000
Western Blot Dilution: 1:5.000 - 1:2.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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: "Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy." in: The EMBO journal, Vol. 40, Issue 14, pp. e105985, (2021) (PubMed).
: "Salinomycin kills cancer stem cells by sequestering iron in lysosomes." in: Nature chemistry, Vol. 9, Issue 10, pp. 1025-1033, (2019) (PubMed).
: "Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9." in: Cell reports, Vol. 20, Issue 10, pp. 2341-2356, (2018) (PubMed).
: "Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy." in: The EMBO journal, Vol. 36, Issue 1, pp. 42-60, (2017) (PubMed).
: "Ferritinophagy via NCOA4 is required for erythropoiesis and is regulated by iron dependent HERC2-mediated proteolysis." in: eLife, Vol. 4, (2016) (PubMed).
: "Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy." in: Nature, Vol. 509, Issue 7498, pp. 105-9, (2014) (PubMed).
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: "Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy." in: The EMBO journal, Vol. 40, Issue 14, pp. e105985, (2021) (PubMed).
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- 抗原
- Ferritin (FE)
- 别名
- Ferritin (FE 产品)
- 背景
- Synonyms: Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH
- 基因ID
- 2495
- UniProt
- P02794
- 途径
- Transition Metal Ion Homeostasis
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