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Cyclin B1 抗体

Rockland 100-401-152 CCNB1 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN97948
Supplier Product No.: 100-401-152
发货至: 中国
  • 抗原 See all Cyclin B1 (CCNB1) 抗体
    Cyclin B1 (CCNB1)
    适用
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    克隆类型
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    多克隆
    标记
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    This Cyclin B1 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
    Supplier Product No.
    100-401-152
    Supplier
    Rockland
    原理
    Cyclin B1 Antibody
    交叉反应 (详细)
    Antiserum will specifically react with a 55-62 kDa Cyclin B1 protein from human, rat and mouse tissue.
    产品特性
    Synonyms: rabbit anti-cyclin B1 antibody, G2/mitotic-specific cyclin-B1
    纯化方法
    This product was prepared from monospecific antiserum by delipidation and defibrination.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.

    Immunogen Type: Native Protein

    Top Product
    Discover our top product CCNB1 Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:500

    Application Note: Anti-Cyclin B1 antibody has been tested by western blot and immunohistochemistry and is suitable for use in ELISA, immunoblotting, immunoprecipitation, immunohistochemistry, and other immunological methods requiring high titer and specificity.   Specific conditions for reactivity and detection of Cyclin B1 should be optimized by the end user.  Expect a band approximately 55-66 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. H23 cells may be used as a positive control.

    Western Blot Dilution: 1:500 - 1:1,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:2,000 - 1:10,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    80 mg/mL
    缓冲液

    Buffer: None

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
    有效期
    12 months
  • Wetzel-Strong, Li, Klein, Nishikimi, Caron: "Epicardial-derived adrenomedullin drives cardiac hyperplasia during embryogenesis." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 243, Issue 2, pp. 243-56, (2014) (PubMed).

  • 抗原
    Cyclin B1 (CCNB1)
    别名
    CCNB1 (CCNB1 产品)
    背景
    Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
    基因ID
    891, 14327896
    UniProt
    P14635
    途径
    Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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