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CDK1 抗体 (C-Term)

Name: CDK1 抗体 (C-Term)
SKU: ABIN97117
Availability: OutOfStock

Rockland 100-401-160 CDK1 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-cdc2 Cyclin Dependent Kinase was used to detect human p34cdc2by western blot in untreated (Contol) and drug treated lysates of MCF-7 cells. (CDK1 抗体 (C-Term))

1 image

产品编号 ABIN97117

Supplier Product No.: 100-401-160

发货至: 中国

Datasheet as PDF

抗原 See all CDK1 抗体

CDK1 (Cyclin-Dependent Kinase 1 (CDK1))
抗原表位
34

23

22

20

17

16

9

9

9

8

8

7

6

4

4

4

4

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
297

169

105

38

33

31

25

20

14

13

11

11

10

9

8

6

4

4

1

1

1

1

1

1

1

1

1

1

1

1
人
宿主
248

59
兔
Compatible Secondaries
克隆类型
236

71
多克隆
标记
166

21

14

12

8

7

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2
This CDK1 antibody is un-conjugated
应用范围
249

109

59

56

54

53

47

44

42

19

18

16

6

4

4

3

2

2

2

2

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Supplier Product No.

100-401-160

Supplier

Rockland

原理

CDC2 p34 Antibody

交叉反应（详细）

Antiserum will specifically react with a 34 kDa cdc2 protein from human, rat and mouse tissue.

产品特性

Synonyms: rabbit anti-Cdc2 antibody, rabbit anti-p34 antibody, Cell Division Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, CDK1, CDC2, CDC28A, CDKN1, P34CDC2

纯化方法

This product was prepared from monospecific antiserum by delipidation and defibrination.

过滤

Sterile filtered

免疫原

Immunogen: CDC2 peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide

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应用备注

Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-p34 cdc2 antibody has been tested in ELISA and western blot and is suitable for immunoblotting, immunohistochemistry immunoprecipitation (as active kinase), and immunoblotting. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. This product is suitable for the detection by immunoblot of human, rat and mouse cdc2. For immunohistochemistry use paraffin embedded tissue.
Western Blot Dilution: 1:250 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:60,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

85 mg/mL

缓冲液

Buffer: None
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
抗原

CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

别名

CDK1 (CDK1 产品)

背景

Background: p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF). The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues. In humans the phosphorylation appears to be performed by p60src. The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins. The phosphorylation activity is coupled to the entry into the M-phase of the cell. p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally. Association with deletion mutants of cyclin halts the M-phase before it is completed.

基因ID

983

NCBI登录号

NP_001307847

UniProt

P06493

途径

Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA