Actin 抗体
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- 抗原 See all Actin 抗体
- Actin
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适用
- 人, 大鼠, 小鼠, 小鸡, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Actin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 交叉反应
- 人, 犬, 大鼠, 小鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Chicken gizzard muscle Actin
- 克隆位点
- C4-actin
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product Actin Primary Antibody
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- 说明
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Related Products: ABIN968537, ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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Differential expression of SNAP-25 family proteins in the mouse brain." in: The Journal of comparative neurology, Vol. 519, Issue 5, pp. 916-32, (2011) (PubMed).
: "Mechanism of actin-based motility." in: Science (New York, N.Y.), Vol. 292, Issue 5521, pp. 1502-6, (2001) (PubMed).
: "Actin-based cell motility and cell locomotion." in: Cell, Vol. 84, Issue 3, pp. 371-9, (1996) (PubMed).
: "Detection of actin and localization of phytochrome in the green alga Mougeotia by monoclonal antibodies." in: Acta histochemica. Supplementband, Vol. 41, Issue 9, pp. 223-30, (1992) (PubMed).
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Differential expression of SNAP-25 family proteins in the mouse brain." in: The Journal of comparative neurology, Vol. 519, Issue 5, pp. 916-32, (2011) (PubMed).
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- 抗原
- Actin
- Abstract
- Actin 产品
- 背景
- Changes in cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton. This reorganization of actin filaments results from interactions between actin and actin-binding proteins. Actin is a 42-kDa protein that is known as G-actin in its monomeric form. Polymerization of G-actin monomers leads to the generation of flexible filaments, 5-9 nm in diameter, called F-actin. F-actin may be organized in linear bundles called stress fibers or in two-dimensional networks. The latter are highly concentrated beneath the plasma membrane and form the actin cortex. Regulation of actin cytoskeletal dynamics occurs through actin-binding proteins. These proteins bind to G- and/or F-actin and regulate various aspects of actin cytoskeletal dynamics, such as polymerization and depolymerization of actin, cross-linking of actin filaments into bundles, interaction of actin-based structures with membranes and other cytoskeletal elements, and locomotion of actin-based structures. Thus, the actin cytoskeleton is a complex matrix consisting of G- and F-actin along with the multitude of interactions between these actin forms and a variety of different types of actin-binding proteins. The C4 monoclonal antibody reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells.
- 分子量
- 42 kDa
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