S100B 抗体 (AA 1-92)
(2 references)-
- 抗原 See all S100B 抗体
- S100B (S100 Calcium Binding Protein B (S100B))
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抗原表位
- AA 1-92
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适用
- 人, 大鼠, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This S100B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)
- 交叉反应
- 大鼠, 人
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse S100B aa. 1-92
- 克隆位点
- 19-S100B
- 亚型
- IgG1
- Top Product
- Discover our top product S100B Primary Antibody
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anti-S100 Calcium Binding Protein B (S100B) (AA 31-92) antibody
S100B 适用: 大鼠 ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人, 大鼠, 小鼠, Cow WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal 4C4-9 unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4A5H7 unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人, 大鼠, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22934 unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人, 大鼠, 小鼠, Cow IHC, StM 宿主: 小鼠 Monoclonal rS100B-1012 unconjugated Recombinant Antibody
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- 应用备注
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Bioimaging:
Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS. - 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20°C.
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: "Concerted regulation of wild-type p53 nuclear accumulation and activation by S100B and calcium-dependent protein kinase C." in: Molecular and cellular biology, Vol. 19, Issue 10, pp. 7168-80, (2000) (PubMed).
: "Organization, sequence, and expression of the murine S100 beta gene. Transcriptional regulation by cell type-specific cis-acting regulatory elements." in: The Journal of biological chemistry, Vol. 268, Issue 27, pp. 20502-11, (1993) (PubMed).
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: "Concerted regulation of wild-type p53 nuclear accumulation and activation by S100B and calcium-dependent protein kinase C." in: Molecular and cellular biology, Vol. 19, Issue 10, pp. 7168-80, (2000) (PubMed).
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- 抗原
- S100B (S100 Calcium Binding Protein B (S100B))
- 别名
- S100B MAB (S100B 产品)
- 背景
- The S100 multigene family consists of a number of small dimeric molecules around 100 residues in length. The original members of this family, S100A and S100B, were initially identified in bovine brain. Other S100 proteins have also been identified, such as S100P from human placenta and S100L from bovine lung. The S100 family consists of at least 10 members which show a cell-type-specific expression pattern. Most S100 proteins are believed to be involved in Ca2+ signaling pathways. S100P consists of 95 amino acids and shares about 50% sequence identity with S100A and S100B, while S100L has been shown to possess 43-47% homology to S100A and S100B. S100B is expressed in the nervous system by glial cells, such as astrocyte, oligodendroglial, ependymal, and microglial cells. The dimeric form of S100B has been implicated in neuronal survival, neurite extension, and astrocyte growth. In addition, S100B expression is induced by stimulations that activate p53, and S100B activity may be involved in PKC-dependent p53 nuclear translocation in early G1 phase. Thus, S100B plays a role in calcium signaling pathways that regulate cell differentiation and cell cycle progression.
- 分子量
- 8 kDa
- 途径
- Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
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