LIG3 抗体 (AA 2-115)
(4 references)-
- 抗原 See all LIG3 抗体
- LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))
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抗原表位
- AA 2-115
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适用
- 人, 小鼠, 大鼠, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This LIG3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 交叉反应
- 犬, 大鼠, 小鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human DNA Ligase III aa. 2-115
- 克隆位点
- 7-D Ligase III
- 亚型
- IgG1
- Top Product
- Discover our top product LIG3 Primary Antibody
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anti-Ligase III, DNA, ATP-Dependent (LIG3) (AA 730-1009) antibody
LIG3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) antibodyLIG3 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (C-Term) antibodyKO Validated LIG3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (AA 809-928) antibodyLIG3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (Internal Region) antibodyLIG3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) antibodyKO Validated LIG3 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (Internal Region) antibodyLIG3 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (AA 823-922) antibodyLIG3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (AA 220-269) antibodyLIG3 适用: 人, 小鼠, 大鼠, 马, 兔, 豚鼠, Cow, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Ligase III, DNA, ATP-Dependent (LIG3) (AA 930-979) antibodyLIG3 适用: 人, 小鼠, 大鼠, 犬, 马, 兔, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 说明
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Related Products: ABIN968537, ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "ATP for the DNA ligation step in base excision repair is generated from poly(ADP-ribose)." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23234-9, (2000) (PubMed).
: "The DNA ligase III zinc finger stimulates binding to DNA secondary structure and promotes end joining." in: Nucleic acids research, Vol. 28, Issue 18, pp. 3558-63, (2000) (PubMed).
: "DNA ligase III is recruited to DNA strand breaks by a zinc finger motif homologous to that of poly(ADP-ribose) polymerase. Identification of two functionally distinct DNA binding regions within DNA ligase III." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21679-87, (1999) (PubMed).
: "Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination." in: Molecular and cellular biology, Vol. 15, Issue 10, pp. 5412-22, (1995) (PubMed).
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: "ATP for the DNA ligation step in base excision repair is generated from poly(ADP-ribose)." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23234-9, (2000) (PubMed).
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- 抗原
- LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))
- 别名
- DNA Ligase III (LIG3 产品)
- 别名
- orf36 antibody, DDBDRAFT_0218523 antibody, DDBDRAFT_0232266 antibody, DDB_0218523 antibody, DDB_0232266 antibody, LIG3 antibody, wu:fb12c02 antibody, wu:fd10b03 antibody, wu:fm79b09 antibody, LIG2 antibody, D11Wsu78e antibody, An02g06890 antibody, AO090003000668 antibody, lig3 antibody, DNA ligase III antibody, BRCT domain-containing protein antibody, DNA ligase 3 antibody, ligase III, DNA, ATP-dependent antibody, ligase III, DNA, ATP-dependent L homeolog antibody, orf36 antibody, lig3 antibody, LIG3 antibody, Lig3 antibody, Bm1_22840 antibody, ANI_1_2644024 antibody, AOR_1_1174154 antibody, LOC100634887 antibody, lig3.L antibody
- 背景
- Cells have evolved DNA repair pathways that are dedicated to the maintenance of DNA integrity. In such pathways, damaged DNA is excised and the resulting gap is filled by DNA polymerase. Human DNA ligases, ligase I, III, and IV, utilize ATP as a co-factor in DNA joining reactions required for base excision and single strand break repair pathways. All DNA ligases contain an RFPR sequence and an active site motif (ASM) on each side of their catalytic domain. The RFPR is required for transfer of an AMP group from the enzyme to the 5'-phosphate terminus of a DNA nick. In addition, DNA ligase III has an N-terminal zinc finger domain (ZFD) that is homoloqous with the zinc fingers found in poly(ADP-ribose) polymerase (PARP). This domain is not required for DNA ligase activity, but enables DNA ligase III to interact with single strand gaps and single strand flaps. During base excision repair (BER), ATP-dependent ligation requires PARP, DNA polymerase beta, and DNA ligase III interaction with XRCC1 within the BER complex. Thus, DNA ligase III may contain unique protein sequences that allow interaction and repair of specific types of DNA damage.
- 分子量
- 103 kDa
- 途径
- DNA Damage Repair
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