PCNT 抗体 (AA 1692-1814)
(3 references)-
- 抗原 See all PCNT 抗体
- PCNT (Pericentrin (PCNT))
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抗原表位
- AA 1692-1814
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适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PCNT antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse pericentrin aa. 1692-1814
- 克隆位点
- 30-Pericentrin
- 亚型
- IgG1
- Top Product
- Discover our top product PCNT Primary Antibody
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anti-Pericentrin (PCNT) (AA 2987-3246) antibody
PCNT 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Pericentrin (PCNT) (AA 250-600) antibodyPCNT 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pericentrin (PCNT) antibodyPCNT 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "Development and distribution of neuronal cilia in mouse neocortex." in: The Journal of comparative neurology, Vol. 520, Issue 4, pp. 848-73, (2012) (PubMed).
: "Cytoplasmic dynein-mediated assembly of pericentrin and gamma tubulin onto centrosomes." in: Molecular biology of the cell, Vol. 11, Issue 6, pp. 2047-56, (2000) (PubMed).
: "Pericentrin, a highly conserved centrosome protein involved in microtubule organization." in: Cell, Vol. 76, Issue 4, pp. 639-50, (1994) (PubMed).
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: "Development and distribution of neuronal cilia in mouse neocortex." in: The Journal of comparative neurology, Vol. 520, Issue 4, pp. 848-73, (2012) (PubMed).
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- 抗原
- PCNT (Pericentrin (PCNT))
- 别名
- Pericentrin (PCNT 产品)
- 别名
- AKAP450 antibody, CG13459 antibody, CG18648 antibody, CG33957 antibody, CG6735 antibody, CP309 antibody, Cp309 antibody, D-PLP antibody, DPLP antibody, Dmel\\CG33957 antibody, Dplp antibody, PACT antibody, PLP antibody, Plp antibody, d-plp antibody, dPLP antibody, dplp antibody, l(3)s2172 antibody, pPlp antibody, KEN antibody, MOPD2 antibody, PCN antibody, PCNT2 antibody, PCNTB antibody, PCTN2 antibody, SCKL4 antibody, DIP2A antibody, AW476095 antibody, C86676 antibody, Pcnt2 antibody, kendrin antibody, m239Asp antibody, m275Asp antibody, pericentrin antibody, Pericentrin-like protein antibody, pericentrin (kendrin) antibody, LOC100055203 antibody, Plp antibody, PCNT antibody, pcnt antibody, Pcnt antibody
- 背景
- Centrosomes and other microtubule-organizing centers are a diverse group of organelles that nucleate and organize microtubules for many celllular processes, such as mitotic spindle formation, organelle tranpsort, and protein localization. Pericentrin is a protein in the pericentriolar material, a filamentous matrix surrounding centrioles, which can organize microtubule spindle formation. The structure of pericentrin includes multiple alpha-helical domains that form coiled-coil domains separated by non-helical, non-coiled regions. It is found in a 3 MDa-complex that includes gamma-tubulin, a form of tubulin that nucleates microtubule formation at the centrosome. Pericentrin exhibits the highest expression in embryonic mouse kidney, thymus, and liver. Injection of pericentrin antibodies in Xenopus oocytes disrupts mitotic and meiotic divisions and blocks microtubule aster formation. In addition, dynein transports both pericentrin and gamma-tubulin to centrosomes along microtubules in Xenopus oocyte extracts. Thus, the complex of pericentrin and gamma-tubulin may be recruited to the centrosome by dynein, where the complex becomes anchored to the centrosome for microtubule nucleating activity. This antibody is routinely tested by western blot analysis.
- 分子量
- 220 kDa
- 途径
- Sensory Perception of Sound, M Phase, SARS-CoV-2 Protein Interactome
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