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Phospholipase C beta 4 抗体 (AA 752-961)

PLCb4 适用: 人, 大鼠, 小鼠, Blow Fly WB, BI, IF 宿主: 小鼠 Monoclonal 56-Phospholipase Cbeta4 unconjugated
产品编号 ABIN968583
发货至: 中国
  • 抗原 See all Phospholipase C beta 4 (PLCb4) 抗体
    Phospholipase C beta 4 (PLCb4)
    抗原表位
    • 1
    • 1
    • 1
    AA 752-961
    适用
    人, 大鼠, 小鼠, Blow Fly
    宿主
    • 10
    • 1
    小鼠
    克隆类型
    • 11
    单克隆
    标记
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Phospholipase C beta 4 antibody is un-conjugated
    应用范围
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), BioImaging (BI), Immunofluorescence (IF)
    交叉反应
    大鼠, 小鼠, Fruit Fly (Drosophila melanogaster)
    产品特性
    1. Please refer to us for technical protocols.
    2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    3. Alexa Fluor is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Human Phospholipase Cbeta4 aa. 752-961
    克隆位点
    56-Phospholipase Cbeta4
    亚型
    IgG1
    Top Product
    Discover our top product PLCb4 Primary Antibody
  • 应用备注
    Bioimaging:
    Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
    Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
    说明

    Related Products: ABIN967389

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    250 μg/mL
    缓冲液
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Store undiluted at -20°C.
  • Kano, Hashimoto, Watanabe, Kurihara, Offermanns, Jiang, Wu, Jun, Shin, Inoue, Simon, Wu: "Phospholipase cbeta4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 26, pp. 15724-9, (1999) (PubMed).

    Kim, Jun, Lee, Kang, Min, Kim, Ryu, Suh, Shin: "Phospholipase C isozymes selectively couple to specific neurotransmitter receptors." in: Nature, Vol. 389, Issue 6648, pp. 290-3, (1997) (PubMed).

    Alvarez, Ghalayini, Xu, Hardcastle, Bhattacharya, Rao, Pettenati, Anderson, Baehr: "cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4 (PLCB4)." in: Genomics, Vol. 29, Issue 1, pp. 53-61, (1996) (PubMed).

    Lee, Park, Lee, Kim, Rhee: "Purification, molecular cloning, and sequencing of phospholipase C-beta 4." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21318-27, (1993) (PubMed).

  • 抗原
    Phospholipase C beta 4 (PLCb4)
    别名
    Phospholipase C beta 4 (PLCb4 产品)
    别名
    ARCND2 antibody, PI-PLC antibody, A930039J07Rik antibody, AI854601 antibody, C230058B11Rik antibody, Beta4aa antibody, RATBETA4AA antibody, phospholipase C beta 4 antibody, phospholipase C, beta 4 antibody, PLCB4 antibody, Plcb4 antibody
    背景
    Phospholipase C (PLC) hydrolyzes inositol phospholipids into diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Multiple distinct PLC isoenzymes have been identified and divided into three structural types: alpha, beta, and gamma. This classification is based primarily on the location of the conserved X and Y domains, whose structural integrity is essential for a functional catalytic core. The activation of PLCbeta isoenzymes is uniquely regulated by G protein subunits, while PLCgamma is activated following phosphorylation by protein tyrosine kinases. The beta subfamily of PLC consists of at least four members: beta1, beta2, beta3, and beta4. PLCbeta4 differs from the other members in that it is not activated by G protein betagamma subunits, it is not found in the liver or kidney, and it is inhibited by ribonucleotides. Various isoforms of PLbetaC4 result from alternative splicing or proteolytic cleavage. PLCbeta4 is expressed in retina and brain and knockout mice display ataxia and abnormalities in metabotropic glutamate receptor function in the cerebellum. Thus, PLCbeta4 is primarily found in neuronal tissues where it is thought to be important in neurotransmitter signaling pathways.
    Synonyms: PLCbeta4
    分子量
    130 kDa
    途径
    WNT signaling, Thyroid Hormone Synthesis, G-protein mediated Events
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