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TOP2B 抗体 (AA 1281-1494)

Name: TOP2B 抗体 (AA 1281-1494)
SKU: ABIN968562
Availability: OutOfStock

TOP2B 适用: 人 WB, IF, BI 宿主: 小鼠 Monoclonal 40-Topo IIbeta unconjugated

Western blot analysis of Topo IIbeta on a Jurkat lysate. (TOP2B 抗体 (AA 1281-1494))

Immunofluorescent staining of A549 cells. (TOP2B 抗体 (AA 1281-1494))

Image no. 3 for anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (AA 1281-1494) antibody (ABIN968562) (TOP2B 抗体 (AA 1281-1494))

3 images

(5 references)

产品编号 ABIN968562

发货至: 中国

Datasheet as PDF

抗原 See all TOP2B 抗体

TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))
抗原表位
15

6

5

3

2

2

1

1

1

1

1

1
AA 1281-1494
适用
48

19

18

5

4

4

3

3

3

1

1
人
宿主
47

2
小鼠
Compatible Secondaries
克隆类型
47

1
单克隆
标记
24

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This TOP2B antibody is un-conjugated
应用范围
20

15

14

13

13

8

7

7

6

3

2

1

1
Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

产品特性

1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human Topo IIbeta aa. 1281-1494

克隆位点

40-Topo IIbeta

亚型

IgG1

Top Product

Discover our top product TOP2B Primary Antibody
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (AA 446-655) antibody
TOP2B 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (N-Term) antibody
TOP2B 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (C-Term) antibody
TOP2B 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (AA 1400-1626) antibody
TOP2B 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (N-Term) antibody
TOP2B 适用: 人, 犬, 猴, Pig WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated

anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (AA 1411-1523) antibody
TOP2B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (C-Term) antibody
TOP2B 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (AA 1439-1488) antibody
TOP2B 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 猴, Pig, 兔, Bat WB 宿主: 兔 Polyclonal unconjugated

anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (AA 1548-1597) antibody
TOP2B 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 猴, Pig, 兔 WB 宿主: 兔 Polyclonal unconjugated

anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (Middle Region) antibody
TOP2B 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

说明

Related Products: ABIN968537, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Shain, Landowski, Dalton et al.: "Adhesion-mediated intracellular redistribution of c-Fas-associated death domain-like IL-1-converting enzyme-like inhibitory protein-long confers resistance to CD95-induced apoptosis in hematopoietic ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 5, pp. 2544-53, (2002) (PubMed).

Suzuki, Tomida, Tsuruo: "Dephosphorylated hypoxia-inducible factor 1alpha as a mediator of p53-dependent apoptosis during hypoxia." in: Oncogene, Vol. 20, Issue 41, pp. 5779-88, (2001) (PubMed).

Brown, Holden, Rahn, Perkins: "Immunohistochemical staining for DNA topoisomerase IIa in Hodgkin's disease." in: American journal of clinical pathology, Vol. 109, Issue 1, pp. 39-44, (1998) (PubMed).

Herzog, Holmes, Tuschong, Ganapathi, Zwelling: "Absence of topoisomerase IIbeta in an amsacrine-resistant human leukemia cell line with mutant topoisomerase IIalpha." in: Cancer research, Vol. 58, Issue 23, pp. 5298-300, (1998) (PubMed).

Tsai-Pflugfelder, Liu, Liu, Tewey, Whang-Peng, Knutsen, Huebner, Croce, Wang: "Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 19, pp. 7177-81, (1988) (PubMed).
抗原

TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

别名

Topo II beta (TOP2B 产品)

别名

TOP2B antibody, D230016L12Rik antibody, Top-2 antibody, TOPIIB antibody, top2beta antibody, DNA topoisomerase II beta antibody, topoisomerase (DNA) II beta antibody, microRNA 4442 antibody, TOP2B antibody, Top2b antibody, MIR4442 antibody

背景

Eukaryotic DNA topoisomerase II, a ubiquitous ATP-dependent type II topoisomerase, is an essential nuclear enzyme in DNA replication and transcription, chromatin segregation, and cell cycle progression. Topoisomerases transiently break a pair of complementary strands in double-stranded DNA to form a gate for the passage of duplex DNA. Two isoforms of DNA topoisomerase II have been identified: topo IIalpha and topo IIß. These exhibit a high degree of homology, except for some divergence in the C-terminal region. Both contain multiple bipartite nuclear localization sequences (NLS) that mediate their subnuclear localization. Topo IIalpha levels rise during late S phase and peak in G2-M, whereas topo IIß levels remain constant throughout the cell cycle. In addition, topo IIalpha is expressed in proliferating cells, while topo IIß is expressed in a wide range of tissues. Although the exact role of these two isoforms during cell proliferation is not known, the differences in cellular expression implicate different physiological roles. Both isoforms may also be important targets for anticancer agents that exert cytotoxicity in proliferating cells via stabilization of a topo II-DNA complex. This antibody is routinely tested by Western blot analysis and immunofluorescent imaging.

分子量

180 kDa