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GRIP1 抗体 (AA 877-1067)

Name: GRIP1 抗体 (AA 877-1067)
SKU: ABIN968471
Availability: OutOfStock

GRIP1 适用: 大鼠 WB, IF, BI 宿主: 小鼠 Monoclonal 32-GRIP unconjugated

Western blot analysis of GRIP on a rat cerebrum lysate. (GRIP1 抗体 (AA 877-1067))

Immunofluorescence staining of undifferentiated SH-SY5Y cells (Human neuroblastoma, ATCC CRL-2266) (left) and differentiated SH-SY5Y cells (right). (GRIP1 抗体 (AA 877-1067))

Image no. 2 for anti-Glutamate Receptor Interacting Protein 1 (GRIP1) (AA 877-1067) antibody (ABIN968471) (GRIP1 抗体 (AA 877-1067))

3 images

(4 references)

产品编号 ABIN968471

发货至: 中国

Datasheet as PDF

抗原 See all GRIP1 抗体

GRIP1 (Glutamate Receptor Interacting Protein 1 (GRIP1))
抗原表位
15

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 877-1067
适用
34

34

19

5

4

3

3

3

2

2

2

2
大鼠
宿主
45

4
小鼠
Compatible Secondaries
克隆类型
46

3
单克隆
标记
26

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GRIP1 antibody is un-conjugated
应用范围
40

13

13

8

7

6

5

4

3

3
Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Rat GRIP aa. 877-1067

克隆位点

32-GRIP

亚型

IgG1

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anti-Glutamate Receptor Interacting Protein 1 (GRIP1) (AA 851-950) antibody
GRIP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A9 unconjugated

anti-Glutamate Receptor Interacting Protein 1 (GRIP1) (Internal Region) antibody
GRIP1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Receptor Interacting Protein 1 (GRIP1) (C-Term) antibody
GRIP1 适用: 人, 小鼠, 大鼠, 豚鼠, 兔, 犬, 马, 斑马鱼, Cow WB 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Receptor Interacting Protein 1 (GRIP1) (AA 1-100) antibody
GRIP1 适用: 小鼠 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Receptor Interacting Protein 1 (GRIP1) (AA 874-1073) antibody
GRIP1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D4 unconjugated

anti-Glutamate Receptor Interacting Protein 1 (GRIP1) (AA 851-950) antibody
GRIP1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Glutamate Receptor Interacting Protein 1 (GRIP1) (N-Term) antibody
GRIP1 适用: 人, 小鼠, 大鼠, 豚鼠, 兔, 马, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Receptor Interacting Protein 1 (GRIP1) (AA 950-999) antibody
GRIP1 适用: 人, 小鼠, 大鼠, 豚鼠, 兔, 犬, 斑马鱼, Cow, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Receptor Interacting Protein 1 (GRIP1) (AA 700-950) antibody
GRIP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Receptor Interacting Protein 1 (GRIP1) (AA 1-190) antibody
GRIP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Bioimaging:
Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

说明

Related Products: ABIN968545, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20°C.
Fallon, Moreau, Croft, Labib, Gu, Fon: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, (2002) (PubMed).

Setou, Seog, Tanaka, Kanai, Takei, Kawagishi, Hirokawa: "Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites." in: Nature, Vol. 417, Issue 6884, pp. 83-7, (2002) (PubMed).

Bowery, Brown: "The cloning of GABA(B) receptors." in: Nature, Vol. 386, Issue 6622, pp. 223-4, (1997) (PubMed).

Dong, OBrien, Fung, Lanahan, Worley, Huganir: "GRIP: a synaptic PDZ domain-containing protein that interacts with AMPA receptors." in: Nature, Vol. 386, Issue 6622, pp. 279-84, (1997) (PubMed).
抗原

GRIP1 (Glutamate Receptor Interacting Protein 1 (GRIP1))

别名

GRIP (GRIP1 产品)

别名

GRIP antibody, 4931400F03Rik antibody, eb antibody, grip antibody, xgrip1 antibody, GRIP1 antibody, si:dkey-244b23.1 antibody, si:dkey-6a5.1 antibody, glutamate receptor interacting protein 1 antibody, glutamate receptor interacting protein 1 S homeolog antibody, glutamate receptor-interacting protein 1 antibody, GRIP1 antibody, Grip1 antibody, grip1.S antibody, LOC525592 antibody, grip1 antibody

背景

Rapid neuronal excitation within the CNS is mediated by the interactions of receptors with their respective neurotransmitters, such as glutamate. Glutamate has a diverse array of receptors that are categorized into two distinct groups: ionotropic and metabotropic. Ionotropic receptors are ligand-gated channels and can be subdivided into two classes: NMDA and AMPA receptors. AMPA receptors are composed of four homologous subunits (GluR1-4) that differentially combine to form a variety of receptor subtypes. Interactions of the subunits with cytoplasmic proteins mediate the transmission of extracellular signals. GRIP (Glutamate Receptor Interacting Protein) specifically interacts with the C-terminus of the GluR2 subunit. GRIP lacks a catalytic domain, but contains seven PDZ domains which are motifs that mediate protein-protein interactions. Since only the fourth and fifth PDZ domains are utilized in interaction with GluR2, it is thought that the remaining PDZ domains interact with other unidentified proteins. Therefore, GRIP functions as an adaptor that links AMPA receptors to cytoskeletal and/or signaling molecules and, in turn, clusters them at the synapse.
Synonyms: Glutamate Receptor Interacting Protein

分子量

130 kDa

途径

Intracellular Steroid Hormone Receptor Signaling Pathway