ITCH 抗体 (AA 114-220)
(1 reference)-
- 抗原 See all ITCH 抗体
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
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抗原表位
- AA 114-220
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适用
- 人, 小鼠, 大鼠, 兔
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ITCH antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 交叉反应
- 大鼠, 人, 兔
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse Itch aa. 114-220
- 克隆位点
- 32-Itch
- 亚型
- IgG1
- Top Product
- Discover our top product ITCH Primary Antibody
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anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (Internal Region) antibody
Verified ITCH 适用: 人 ELISA, WB, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) antibodyKO Validated ITCH 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (Tyr420) antibodyITCH 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 29-303) antibodyITCH 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 92-190) antibodyITCH 适用: 人 ELISA, IF 宿主: 小鼠 Monoclonal 1B8 unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 50-150) antibodyKO Validated ITCH 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) antibodyITCH 适用: 人 ELISA, WB 宿主: 兔 Monoclonal 9C3 unconjugated Recombinant Antibody
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (pTyr420) antibodyITCH 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 143-192) antibodyITCH 适用: 人, Cow, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 410-437), (Middle Region) antibodyITCH 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 说明
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Related Products: ABIN968548, ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "The itchy locus encodes a novel ubiquitin protein ligase that is disrupted in a18H mice." in: Nature genetics, Vol. 18, Issue 2, pp. 143-6, (1998) (PubMed).
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: "The itchy locus encodes a novel ubiquitin protein ligase that is disrupted in a18H mice." in: Nature genetics, Vol. 18, Issue 2, pp. 143-6, (1998) (PubMed).
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- 抗原
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
- 别名
- Itch (ITCH 产品)
- 别名
- AIF4 antibody, AIP4 antibody, NAPP1 antibody, dJ468O1.1 antibody, 6720481N21Rik antibody, 8030492O04Rik antibody, A130065M08 antibody, C230047C07Rik antibody, itchy E3 ubiquitin protein ligase antibody, itchy, E3 ubiquitin protein ligase antibody, ITCH antibody, Itch antibody
- 背景
- Maintenance of cellular function requires timely and selective degradation of key regulatory proteins. For example, progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins via the ubiquitin pathway. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an epsilon-NH2-Lys in a target protein. Ubiquitin-conjugated proteins are recognized and degraded by the 26S proteasome. Ubiquitination requires ubiquitin-activating enzyme E1, ubiquitin-conjugating enzymes E2, and ubiquitin ligases E3. The direction of ubiquitin transfer is from E1 to E2 and from E2 to E3. Itch, a novel E3 ubiquitin ligase, is absent in the Non-agouti-lethal 18H mice. These mice develop immunological, inflammatory, epithelial, and hematopoietic diseases. Itch contains four WW protein interaction domains, which bind to proline-rich sequences in a fashion similar to SH3 domains. In addition, Itch contains a C-terminal Hect domain, which is conserved in the E3 family of ubiquitin ligases. Thus, Itch is important in the ubiquitin-dependent protein degradation occurring in normal hematopoiesis and inflammation.
- 分子量
- 113 kDa
- 途径
- Activation of Innate immune Response, CXCR4-mediated Signaling Events
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