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RAD50 抗体 (AA 672-786)

Name: RAD50 抗体 (AA 672-786)
SKU: ABIN968313
Availability: OutOfStock

RAD50 适用: 人 WB, IF 宿主: 小鼠 Monoclonal 13-RAD50 unconjugated

Western blot analysis of RAD50 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). (RAD50 抗体 (AA 672-786))

Immunofluorescence staining of human endothelial cells. (RAD50 抗体 (AA 672-786))

Image no. 3 for anti-RAD50 Homolog (S. Cerevisiae) (RAD50) (AA 672-786) antibody (ABIN968313) (RAD50 抗体 (AA 672-786))

Western Blotting (WB) image for anti-RAD50 Homolog (S. Cerevisiae) (RAD50) (AA 672-786) antibody (ABIN968313) (RAD50 抗体 (AA 672-786))

4 images

(5 references)

产品编号 ABIN968313

发货至: 中国

Datasheet as PDF

抗原 See all RAD50 抗体

RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))
抗原表位
16

8

8

7

6

5

4

3

3

3

2

2

2

2

1

1

1

1
AA 672-786
适用
55

51

19

5

5

4

3

2

2

1

1

1

1

1
人
宿主
66

5
小鼠
Compatible Secondaries
克隆类型
65

6
单克隆
标记
35

7

4

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This RAD50 antibody is un-conjugated
应用范围
61

24

17

17

13

13

10

10

8

5

3

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF)

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human RAD50 aa. 672-786

克隆位点

13-RAD50

亚型

IgG1

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anti-RAD50 Homolog (S. Cerevisiae) (RAD50) antibody
RAD50 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-RAD50 Homolog (S. Cerevisiae) (RAD50) (AA 228-359) antibody
RAD50 适用: 人, 大鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3D2G10 unconjugated

anti-RAD50 Homolog (S. Cerevisiae) (RAD50) (AA 1-100) antibody
RAD50 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-RAD50 Homolog (S. Cerevisiae) (RAD50) (Internal Region) antibody
RAD50 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-RAD50 Homolog (S. Cerevisiae) (RAD50) (AA 228-359) antibody
RAD50 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5A8E5 unconjugated

anti-RAD50 Homolog (S. Cerevisiae) (RAD50) (AA 1-234) antibody
RAD50 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-RAD50 Homolog (S. Cerevisiae) (RAD50) antibody
RAD50 适用: 人 WB 宿主: 兔 Monoclonal unconjugated

anti-RAD50 Homolog (S. Cerevisiae) (RAD50) (AA 817-872) antibody
RAD50 适用: 人, 小鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-RAD50 Homolog (S. Cerevisiae) (RAD50) (AA 650-730) antibody
RAD50 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-RAD50 Homolog (S. Cerevisiae) (RAD50) (AA 700-750) antibody
RAD50 适用: 人, 小鼠, Cow, 兔, 犬 IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN968537, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Huber, Yang, Sarkisian, Master, Deng, Chodosh: "Impaired DNA damage response in cells expressing an exon 11-deleted murine Brca1 variant that localizes to nuclear foci." in: Molecular and cellular biology, Vol. 21, Issue 12, pp. 4005-15, (2001) (PubMed).

Ohta, Nicolas, Furuse, Nabetani, Ogawa, Shibata: "Mutations in the MRE11, RAD50, XRS2, and MRE2 genes alter chromatin configuration at meiotic DNA double-stranded break sites in premeiotic and meiotic cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 646-51, (1998) (PubMed).

Trujillo, Yuan, Lee, Sung: "Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21447-50, (1998) (PubMed).

Dolganov, Maser, Novikov, Tosto, Chong, Bressan, Petrini: "Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 4832-41, (1996) (PubMed).
抗原

RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

别名

Rad50 (RAD50 产品)

别名

NBSLD antibody, RAD502 antibody, hRad50 antibody, Mrell antibody, Rad50l antibody, 6339 antibody, CG6339 antibody, Dmel\\CG6339 antibody, Dmrad50 antibody, RAD50 antibody, Rad50 antibody, ATRAD50 antibody, DNA REPAIR-RECOMBINATION PROTEIN antibody, F22D22.28 antibody, F22D22_28 antibody, SPAP4C9.01c antibody, im:6906849 antibody, si:ch211-79h18.3 antibody, RAD50 double strand break repair protein antibody, CG6339 gene product from transcript CG6339-RE antibody, RAD50 homolog, double strand break repair protein L homeolog antibody, rad50 antibody, DNA repair protein RAD50 antibody, RAD50 homolog, double strand break repair protein antibody, DNA repair-recombination protein (RAD50) antibody, MRX complex DNA-binding subunit antibody, DNA repair protein Rad50 antibody, RAD50 recombination protein antibody, Rad50 DNA repair/recombination protein antibody, DNA repair protein rad-50 antibody, RAD50 antibody, Rad50 antibody, rad50 antibody, rad50.L antibody, LOC658288 antibody, LOC726642 antibody, rad-50 antibody

背景

DNA double-strand breaks (DSBs) are generated during intrinsic eukaryotic DNA recombination events such as assembly of antigen receptor genes, meiotic and miotic recombination. DNA DSB repair proteins are also required to repair breaks induced by extrinsic factors such as ionizing radiation and mutagenic chemicals. Originally identified in S. cerevisiae, Rad50 is one of a group of genes, designated as the Rad52 epistasis group, whose products mediate DSB repair. Many of these genes, including Rad50, are conserved in humans and are thought to have a similar function to their S. cerevisiae counterparts. In yeast, a multiprotein complex of Rad50, MRE11, and XRS2 has been implicated in the nucleocytic processing of DSBs. In humans, Rad50 and MRE11 complex with up to three additional proteins (95 kDa, 200 kDa, and 350 kDa). The 95 kDa species is thought to be human XRS2, although a separate report has identified it as Nibrin, the product of the gene mutated in Nijmegen breakage syndrome. The Rad50-MRE11-p95 complex possess endonuclease and 3' to 5' exonuclease activity. Thus, human Rad50 functions in a multiprotein complex to mediate the repair of DSBs in the human genome. This antibody is routinely tested by western blot analysis.

分子量

154 kDa

途径

DNA Damage Repair, Protein targeting to Nucleus