MCM2 抗体 (AA 725-888)
(5 references)-
- 抗原 See all MCM2 抗体
- MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))
-
抗原表位
- AA 725-888
-
适用
- 人, 小鼠, 大鼠, 犬, 小鸡
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This MCM2 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
- 交叉反应
- 小鸡, 犬, 小鼠, 大鼠
- 产品特性
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human BM28 aa. 725-888
- 克隆位点
- 46-BM28
- 亚型
- IgG1
- Top Product
- Discover our top product MCM2 Primary Antibody
-
anti-Minichromosome Maintenance Complex Component 2 (MCM2) antibody
MCM2 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 2B3 unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) antibodyMCM2 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1E7 unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 805-904) antibodyMCM2 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 6A8 unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) antibodyMCM2 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 884-904) antibodyMCM2 适用: 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (N-Term) antibodyMCM2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 360-700) antibodyMCM2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 889-904), (C-Term) antibodyMCM2 适用: 人, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 884-904), (C-Term) antibodyMCM2 适用: 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 649-677) antibodyMCM2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
-
- 说明
-
Related Products: ABIN968536, ABIN967389
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
-
-
: "Analysis of Cdc6 function in the assembly of mammalian prereplication complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 3, pp. 1347-52, (2002) (PubMed).
: "Cell type-specific responses of human cells to inhibition of replication licensing." in: Oncogene, Vol. 21, Issue 43, pp. 6624-32, (2002) (PubMed).
: "Binding of human minichromosome maintenance proteins with histone H3." in: The Journal of biological chemistry, Vol. 271, Issue 39, pp. 24115-22, (1996) (PubMed).
: "BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication." in: The Journal of cell biology, Vol. 129, Issue 6, pp. 1433-45, (1995) (PubMed).
: "A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division." in: Journal of cell science, Vol. 107 ( Pt 1), pp. 253-65, (1994) (PubMed).
-
: "Analysis of Cdc6 function in the assembly of mammalian prereplication complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 3, pp. 1347-52, (2002) (PubMed).
-
- 抗原
- MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))
- 别名
- BM28 (MCM2 产品)
- 别名
- CG7538 antibody, DmMCM2 antibody, DmMcm2 antibody, DmeMCM2 antibody, Dmel\\CG7538 antibody, MCM2 antibody, MCM2-7 antibody, MCM2_DROME antibody, McM2 antibody, PCR3 antibody, dMCM2 antibody, l(3)rL074 antibody, XMcm2 antibody, bm28 antibody, ccnl1 antibody, cdc19 antibody, cdcl1 antibody, mitotin antibody, cb737 antibody, chunp6905 antibody, BM28 antibody, CCNL1 antibody, CDCL1 antibody, D3S3194 antibody, MITOTIN antibody, MCM7 antibody, AA959861 antibody, AW476101 antibody, Mcmd2 antibody, mKIAA0030 antibody, Minichromosome maintenance 2 antibody, minichromosome maintenance complex component 2 antibody, minichromosome maintenance complex component 2 L homeolog antibody, Mcm2 antibody, mcm2 antibody, MCM2 antibody, mcm2.L antibody
- 背景
- BM28 is a phosphoprotein that migrates at 125kDa in SDS PAGE with a hyperphosphorylated form migrating as a slightly slower band. The ratio of the phosphorylation states alters with the phases of cell cycle - in M, the fast phosphorylated BM28 is the major form found, while in G1 phase, the slow, hyperphosphorylated BM28 predominates. The cellular localization of BM28 is also cell cycle dependent. In G1, most of the BM28 is chromatin bound, whereas, by M phase, the BM28 is still nuclear, but not associated with the DNA. BM28 is necessary for both entry into S phase and cell division as determined by microinjection inhibition experiments. Microinjection of a BM28 antibody into synchronised cells in G1 phase inhibits DNA replication. When injected during S phase or later, no effect on DNA replication is seen, but cell division is inhibited.
- 分子量
- 125 kDa
- 途径
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
-
