SKP1 抗体 (AA 4-158)
(4 references)-
- 抗原 See all SKP1 抗体
- SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))
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抗原表位
- AA 4-158
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适用
- 人, 小鼠, 大鼠, 小鸡, 犬, Frog
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SKP1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), BioImaging (BI)
- 交叉反应
- 小鸡, 犬, Frog, 小鼠, 大鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human p19 [Skp1] aa. 4-158
- 克隆位点
- 52-p19 [Skp1]
- 亚型
- IgG1
- Top Product
- Discover our top product SKP1 Primary Antibody
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anti-S-Phase Kinase-Associated Protein 1 (SKP1) antibody
SKP1 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 4E11 unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 53-160) antibodySKP1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1H8 unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (Internal Region) antibodySKP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) antibodySKP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 1-163) antibodySKP1 适用: 人, 大鼠, 猴, 小鸡, Cow, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) antibodySKP1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 1-163) antibodySKP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 1-160) antibodySKP1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 81-163) antibodySKP1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 2-163) antibodySKP1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument. - 说明
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Related Products: ABIN967389, ABIN968533
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20°C.
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: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).
: "Role of the F-box protein Skp2 in adhesion-dependent cell cycle progression." in: The Journal of cell biology, Vol. 153, Issue 7, pp. 1381-90, (2001) (PubMed).
: "beta-Trcp couples beta-catenin phosphorylation-degradation and regulates Xenopus axis formation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 11, pp. 6273-8, (1999) (PubMed).
: "p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase." in: Cell, Vol. 82, Issue 6, pp. 915-25, (1995) (PubMed).
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: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).
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- 抗原
- SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))
- 别名
- p19 Skp1 (SKP1 产品)
- 别名
- EMC19 antibody, OCP-II antibody, OCP2 antibody, SKP1A antibody, TCEB1L antibody, p19A antibody, 15kDa antibody, 2610043E24Rik antibody, 2610206H23Rik antibody, SKP1 antibody, Tceb1l antibody, p19Skp1 antibody, Skp1a antibody, skp1a antibody, zgc:73186 antibody, ocp2 antibody, emc19 antibody, ocp-ii antibody, tceb1l antibody, ARABIDOPSIS SKP1 HOMOLOGUE 1 antibody, ASK1 antibody, ATSKP1 antibody, S phase kinase-associated protein 1 antibody, T4O12.17 antibody, T4O12_17 antibody, UFO INTERACTING PROTEIN 1 antibody, UIP1 antibody, ocp-2 antibody, S-phase kinase associated protein 1 antibody, S-phase kinase-associated protein 1 antibody, S-phase kinase-associated protein 1 pseudogene antibody, S-phase kinase-associated protein 1A antibody, S-phase kinase-associated protein 1 S homeolog antibody, S phase kinase-associated protein 1 antibody, SKP1 antibody, skp1 antibody, LOC100340043 antibody, Skp1a antibody, Skp1 antibody, skp1.S antibody
- 背景
- In normal cells, Cyclin A and Cdk2 are components of an active quaternary complex of proteins that includes Cip1 (p21) and PCNA (proliferating cell nuclear antigen). This Cyclin A-Cdk2 complex is required during S phase. In transformed cells, Cyclin A and Cdk2 form complexes with three different proteins: p9, p19, and p45. p19 [Skp1], named for S phase kinase-dependent protein, and p45 [Skp2] are essential elements in the S phase kinase complex that is present in many transformed cell lines. p19 [Skp1] binds to Cyclin A-Cdk2 only when in combination with p45 [Skp2]. The mechanism that mediates p19 and p45 association with Cyclin A-Cdk2 is not yet known.
- 分子量
- 19 kDa
- 途径
- Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases
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