CDC27 抗体 (AA 145-343)
(4 references)-
- 抗原 See all CDC27 抗体
- CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
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抗原表位
- AA 145-343
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适用
- 人, 大鼠, 小鼠, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CDC27 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
- 交叉反应
- 犬, 大鼠, 小鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human CDC27 aa. 145-343
- 克隆位点
- 35-CDC27
- 亚型
- IgG2b
- Top Product
- Discover our top product CDC27 Primary Antibody
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anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 551-830) antibody
CDC27 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) antibodyCDC27 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 5C12 unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 258-431) antibodyCDC27 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 1-830) antibodyCDC27 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 395-444) antibodyCDC27 适用: 人, 大鼠, Cow, 马, 兔, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 422-430) antibodyCDC27 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 239-249), (pThr244) antibodyCDC27 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (Middle Region) antibodyCDC27 适用: 人, 大鼠, 犬, Cow, 马, 兔, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 814-823), (C-Term) antibodyCDC27 适用: 人, 大鼠, 小鼠, 犬, Cow, 非洲爪蟾 WB, IP, IHC (p), EIA 宿主: 小鼠 Monoclonal AF3-1 unconjugated
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- 说明
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Related Products: ABIN968535, ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).
: "Mitotic regulation of the APC activator proteins CDC20 and CDH1." in: Molecular biology of the cell, Vol. 11, Issue 5, pp. 1555-69, (2000) (PubMed).
: "CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition." in: Cell, Vol. 81, Issue 2, pp. 261-8, (1995) (PubMed).
: "Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 21, pp. 10031-5, (1993) (PubMed).
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: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).
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- 抗原
- CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
- 别名
- Cdc27 (CDC27 产品)
- 别名
- ANAPC3 antibody, APC3 antibody, CDC27Hs antibody, D0S1430E antibody, D17S978E antibody, HNUC antibody, NUC2 antibody, wu:fb54g06 antibody, wu:fc88c12 antibody, AI452358 antibody, BC023187 antibody, CDC27 antibody, NV10210 antibody, cell division cycle 27 antibody, cell division cycle 27 S homeolog antibody, cell division cycle protein 27 homolog antibody, anaphase-promoting complex subunit Apc3 antibody, CDC27 antibody, cdc27 antibody, Cdc27 antibody, cdc27.S antibody, LOC100123129 antibody, nuc2 antibody
- 背景
- CDC16 and CDC27 were discovered in yeast as genes essential for the G2/M transition of the cell cycle. The human homologues of CDC16 and CDC27 were cloned and found to encode proteins of 72kDa and 97kDa, respectively. Biochemical and subcellular localization experiments have shown that CDC16 and CDC27 are novel components of the mitotic spindle centrosome in human cells. In HeLa cells, there are about 100,000 copies of each protein per cell. Although its precise role during mitosis remains to be determined, CDC16 appears to be required for cyclin degradation in yeast. Wild type CDC27 is required for the initiation of anaphase. Immunofluorescence studies have shown that both proteins are components of the centrosome in the absence of spindle fibers.
- 分子量
- 97 kDa
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