DAP Kinase 1 抗体 (AA 694-947)
(2 references)-
- 抗原 See all DAP Kinase 1 (DAPK1) 抗体
- DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))
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抗原表位
- AA 694-947
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This DAP Kinase 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human DAP Kinase aa. 694-947
- 克隆位点
- 17-DAP Kinase
- 亚型
- IgG1
- Top Product
- Discover our top product DAPK1 Primary Antibody
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anti-Death-Associated Protein Kinase 1 (DAPK1) (AA 1360-1389), (C-Term) antibody
DAPK1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB3033 unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (AA 1331-1430) antibodyDAPK1 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (AA 1211-1310) antibodyDAPK1 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 2E7 unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (Internal Region) antibodyDAPK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) antibodyDAPK1 适用: 人, 小鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) antibodyDAPK1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (Ser296) antibodyDAPK1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (N-Term) antibodyDAPK1 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (AA 1141-1430) antibodyDAPK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) antibodyDAPK1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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- 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
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: "DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity." in: The EMBO journal, Vol. 16, Issue 5, pp. 998-1008, (1997) (PubMed).
: "Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death." in: Genes & development, Vol. 9, Issue 1, pp. 15-30, (1995) (PubMed).
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: "DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity." in: The EMBO journal, Vol. 16, Issue 5, pp. 998-1008, (1997) (PubMed).
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- 抗原
- DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))
- 别名
- DAP Kinase (DAPK1 产品)
- 别名
- DAPK1 antibody, MGC81366 antibody, si:ch211-66i11.1 antibody, wu:fj09c03 antibody, dapk antibody, MGC83745 antibody, 2310039H24Rik antibody, 2810425C21Rik antibody, AI642003 antibody, D13Ucla1 antibody, DAP-Kinase antibody, DAPK antibody, death-associated protein kinase 1 antibody, death associated protein kinase 1 L homeolog antibody, death associated protein kinase 1 antibody, death associated protein kinase 1 S homeolog antibody, LOC427465 antibody, dapk1.L antibody, DAPK1 antibody, dapk1 antibody, dapk1.S antibody, Dapk1 antibody
- 背景
- Chronic exposure to extracellular signals such as interferons induce the inhibition of cell proliferation followed by cell death. The gene for DAP Kinase (Death Associated Protein Kinase) was identified using a novel approach named Technical Knockout. Briefly, Hela cells were transfected with an antisense cDNA expression library, then exposed to Interferon-gamma. The surviving cells, with their antisense cDNAs, were rescued and the protecting genes isolated. The DAP Kinase gene encodes a protein of 1423 amino acids, a molecular weight of 160kDa, a kinase domain at its amino terminal region, ankyrin repeats in the middle region, and a death domain at the extreme C-terminus. DAP Kinase phosphorylates at Ser/Thr residues in a Ca2+/Calmodulin-dependent fashion. It has been demonstrated that Ca2+/Calmodulin binds directly to DAP Kinase at its amino terminal region. In addition, immunostaining studies localized DAP Kinase in association with the actin filaments where it may phosphorylate myosin light chain. Thus, this novel cytoskeletal and Ca2+/Calmodulin-dependent protein kinase plays a role in interferon-gamma-induced cell death. This antibody is routinely tested by western blot analysis.
- 分子量
- 160 kDa
- 途径
- MAPK Pathway, Interferon-gamma Pathway
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