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CDKN1B 抗体 (AA 1-197)

Name: CDKN1B 抗体 (AA 1-197)
SKU: ABIN967850
Availability: OutOfStock

CDKN1B 适用: 人, 小鼠, 大鼠, 犬, 小鸡, Frog WB, IHC, IF, IP 宿主: 小鼠 Monoclonal 57-Kip1-p27 unconjugated

Western blot analysis of p27[Kip1] on HeLa cell lysate. (CDKN1B 抗体 (AA 1-197))

Immunofluorescent staining of HeLa cells. (CDKN1B 抗体 (AA 1-197))

2 images

(7 references)

产品编号 ABIN967850

发货至: 中国

Datasheet as PDF

抗原 See all CDKN1B 抗体

CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
抗原表位
29

26

20

18

16

16

10

10

9

8

8

8

8

8

7

7

5

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-197
适用
266

122

103

26

6

6

3

3

3

3

2

1

1
人, 小鼠, 大鼠, 犬, 小鸡, Frog
宿主
231

48

2

1

1
小鼠
Compatible Secondaries
克隆类型
226

57
单克隆
标记
134

23

17

16

16

14

4

4

4

4

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
This CDKN1B antibody is un-conjugated
应用范围
197

130

67

60

48

41

33

26

19

16

16

11

7

7

3

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

交叉反应

人, 犬, 大鼠, 小鸡, Frog

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse Kip1 aa. 1-197

克隆位点

57-Kip1-p27

亚型

IgG1

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anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 150-198) antibody
CDKN1B 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 4B4-E6 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
KO Validated CDKN1B 适用: 人 WB, IHC, IF, IHC (p), IP, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B 适用: 人 IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal SX53G8 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 1G2 unconjugated Recombinant Antibody

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B 适用: 小鼠 WB, IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal DCS-72-F6 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr187) antibody
CDKN1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-100) antibody
CDKN1B 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN968535, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Guo, Packard, Krolewski, Harris, Manglapus, Schwob: "Expression of pax6 and sox2 in adult olfactory epithelium." in: The Journal of comparative neurology, Vol. 518, Issue 21, pp. 4395-418, (2011) (PubMed).

Martín-Ibáñez, Crespo, Urbán, Sergent-Tanguy, Herranz, Jaumot, Valiente, Long, Pineda, Andreu, Rubenstein, Marín, Georgopoulos, Mengod, Fariñas, Bachs, Alberch, Canals: "Ikaros-1 couples cell cycle arrest of late striatal precursors with neurogenesis of enkephalinergic neurons." in: The Journal of comparative neurology, Vol. 518, Issue 3, pp. 329-51, (2010) (PubMed).

Poché, Furuta, Chaboissier, Schedl, Behringer: "Sox9 is expressed in mouse multipotent retinal progenitor cells and functions in Müller glial cell development." in: The Journal of comparative neurology, Vol. 510, Issue 3, pp. 237-50, (2008) (PubMed).

Barnouin, Dubuisson, Child, Fernandez de Mattos, Glassford, Medema, Mann, Lam: "H2O2 induces a transient multi-phase cell cycle arrest in mouse fibroblasts through modulating cyclin D and p21Cip1 expression." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13761-70, (2002) (PubMed).

Williamson, Dadmanesh, Koeffler: "BRCA1 transactivates the cyclin-dependent kinase inhibitor p27(Kip1)." in: Oncogene, Vol. 21, Issue 20, pp. 3199-206, (2002) (PubMed).

Frey, Clark, Leontieva, Uronis, Black, Black: "Protein kinase C signaling mediates a program of cell cycle withdrawal in the intestinal epithelium." in: The Journal of cell biology, Vol. 151, Issue 4, pp. 763-78, (2000) (PubMed).

Polyak, Kato, Solomon, Sherr, Massague, Roberts, Koff: "p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest." in: Genes & development, Vol. 8, Issue 1, pp. 9-22, (1994) (PubMed).
抗原

CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

别名

p27 Kip1 (CDKN1B 产品)

别名

CDKN4 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, P27KIP1 antibody, cdkn1b antibody, sb:cb611 antibody, wu:fb63g10 antibody, wu:fb64g10 antibody, wu:fe18e03 antibody, CDKN1B antibody, Kip1 antibody, p27 antibody, FAM14D antibody, ISG12 antibody, ISG12A antibody, P27 antibody, Cdki1b antibody, AA408329 antibody, AI843786 antibody, p27Kip1 antibody, cdkn1bl antibody, cyclin dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Bb antibody, interferon alpha inducible protein 27 antibody, cyclin-dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Ba antibody, CDKN1B antibody, cdkn1bb antibody, IFI27 antibody, Cdkn1b antibody, cdkn1ba antibody

背景

Kip1 is a cyclin-dependent kinase (cdk) inhibitor that was identified as a result of its role in TGFbeta-induced G1 phase arrest and cell-cell contact. In vitro , p27 [Kip1] binds tightly to Cyclin D-Cdk4, Cyclin E-Cdk2, and Cyclin A-Cdk2 complexes and inhibits their activity. In normal cells, Kip1 is sequestered and its activity gradually decreases as cells reach S phase. The addition of TGF-beta in early G1 blocks this decline in activity, by preventing the sequestration of Kip1. TGF-beta also reduces the levels of Cdk4. Kip1 preferentially binds Cyclin D-Cdk4, but the lower levels of Cdk4 in TGF-beta treated cells allow Kip1 to be available for binding to Cyclin E-Cdk2 and Cyclin A-Cdk2. Kip1 is structurally related to Cip1/WAF1, having a similar 60 amino acid sequence in the N-terminal region. A 52 amino acid Kip1 peptide (residues 28-79) from this region is sufficient to inhibit cdk activity in vitro. This antibody is routinely tested by western blot analysis.

分子量

27 kDa

途径

Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy