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PKC alpha 抗体 (AA 270-427)

Name: PKC alpha 抗体 (AA 270-427)
SKU: ABIN967750
Availability: OutOfStock

PKCa 适用: 人, 小鼠, 大鼠, 犬, 小鸡, Frog WB, IHC, IP, IF 宿主: 小鼠 Monoclonal 3-PKCalpha unconjugated

Western blot analysis of PKCalpha on a rat cerebrum lysate. (PKC alpha 抗体 (AA 270-427))

Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721). (PKC alpha 抗体 (AA 270-427))

2 images

(9 references)

产品编号 ABIN967750

发货至: 中国

Datasheet as PDF

抗原 See all PKC alpha (PKCa) 抗体

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
抗原表位
23

21

16

12

10

9

8

7

7

6

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 270-427
适用
162

89

66

5

5

3

3

2

2

1

1

1

1
人, 小鼠, 大鼠, 犬, 小鸡, Frog
宿主
160

10

1
小鼠
Compatible Secondaries
克隆类型
141

29
单克隆
标记
82

14

10

6

6

6

6

6

6

6

4

4

2

2

1

1

1

1

1

1

1

1

1

1

1
This PKC alpha antibody is un-conjugated
应用范围
114

66

53

22

18

16

16

15

14

7

5

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

交叉反应

大鼠, 小鼠, 小鸡, 犬, Frog

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human PKCalpha aa. 270-427

克隆位点

3-PKCalpha

亚型

IgG2b kappa

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PKCa 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB01012 unconjugated

anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (N-Term) antibody
PKCa 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

说明

Related Products: ABIN968545, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Sakagami, Katsumata, Hara, Tamaki, Watanabe, Harvey, Fukaya: "Distinct synaptic localization patterns of brefeldin A-resistant guanine nucleotide exchange factors BRAG2 and BRAG3 in the mouse retina." in: The Journal of comparative neurology, Vol. 521, Issue 4, pp. 860-76, (2013) (PubMed).

Blom, Giove, Deshpande, Eldred: "Characterization of nitric oxide signaling pathways in the mouse retina." in: The Journal of comparative neurology, Vol. 520, Issue 18, pp. 4204-17, (2013) (PubMed).

Besalduch, Tomàs, Santafé, Garcia, Tomàs, Lanuza: "Synaptic activity-related classical protein kinase C isoform localization in the adult rat neuromuscular synapse." in: The Journal of comparative neurology, Vol. 518, Issue 2, pp. 211-28, (2009) (PubMed).

Fujino, Wu, Lin, Phillips, Nedivi: "cpg15 and cpg15-2 constitute a family of activity-regulated ligands expressed differentially in the nervous system to promote neurite growth and neuronal survival." in: The Journal of comparative neurology, Vol. 507, Issue 5, pp. 1831-45, (2008) (PubMed).

Powner, Hodgkin, Wakelam: "Antigen-stimulated activation of phospholipase D1b by Rac1, ARF6, and PKCalpha in RBL-2H3 cells." in: Molecular biology of the cell, Vol. 13, Issue 4, pp. 1252-62, (2002) (PubMed).

Wagner, Fowler, Takemoto: "The interaction and phosphorylation of tropomodulin by protein kinase Calpha in N/N 1003A lens epithelial cells." in: Molecular vision, Vol. 8, pp. 394-406, (2002) (PubMed).

Zhang, Bontrager, Hemler: "Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25005-13, (2001) (PubMed).

Smart, Ying, Anderson: "Hormonal regulation of caveolae internalization." in: The Journal of cell biology, Vol. 131, Issue 4, pp. 929-38, (1996) (PubMed).

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, (1988) (PubMed).
抗原

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

别名

PKC alpha (PKCa 产品)

别名

pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody

背景

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members ( delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. Overexpression of PKCalpha has been reported to lead to an enhanced growth rate and induces the phosphorylation of two cellular proteins of 52 kDa and 90 kDa. This antibody is routinely tested by western blot analysis.
This antibody has been reported to also crossreact with PKCbeta.
Synonyms: Protein Kinase Calpha

分子量

82 kDa

途径

WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling