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CDK4 抗体 (N-Term)

Name: CDK4 抗体 (N-Term)
SKU: ABIN967650
Availability: OutOfStock

CDK4 适用: 人, 小鼠, 大鼠, 犬 WB, IP, IHC (fro), BI 宿主: 小鼠 Monoclonal DCS-35 unconjugated

Western blot analysis of Cdk4. (CDK4 抗体 (N-Term))

Image no. 1 for anti-Cyclin-Dependent Kinase 4 (CDK4) (N-Term) antibody (ABIN967650) (CDK4 抗体 (N-Term))

Immunofluorescent staining of HeLa (ATCC CCL-2) cells. (CDK4 抗体 (N-Term))

3 images

(2 references)

产品编号 ABIN967650

发货至: 中国

Datasheet as PDF

抗原 See all CDK4 抗体

CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
抗原表位
19

16

15

9

9

8

5

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1
N-Term
适用
108

66

51

19

8

6

4

3

2

1

1

1
人, 小鼠, 大鼠, 犬
宿主
94

34

2

1
小鼠
Compatible Secondaries
克隆类型
97

33
单克隆
标记
76

10

9

5

5

4

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CDK4 antibody is un-conjugated
应用范围
108

48

32

24

16

16

15

13

13

11

4

4

2

2

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), BioImaging (BI)

品牌

BD Pharmingen™

交叉反应

犬, 大鼠, 小鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Triton is a trademark of the Dow Chemical Company.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human Cdk4

克隆位点

DCS-35

亚型

IgG1 kappa

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anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 272-303), (C-Term) antibody
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anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 272-303) antibody
CDK4 适用: 人, 大鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

说明

Related Products: ABIN967389

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Johnson, Walker: "Cyclins and cell cycle checkpoints." in: Annual review of pharmacology and toxicology, Vol. 39, pp. 295-312, (1999) (PubMed).

Matsushime, Ewen, Strom, Kato, Hanks, Roussel, Sherr: "Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins." in: Cell, Vol. 71, Issue 2, pp. 323-34, (1992) (PubMed).
抗原

CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

别名

Cdk4 (CDK4 产品)

别名

zgc:153726 antibody, CMM3 antibody, PSK-J3 antibody, Crk3 antibody, 8-6 antibody, CDK4 antibody, CDK4/6 antibody, CG5072 antibody, Cdk4/6 antibody, DmCdk4 antibody, Dmel\\CG5072 antibody, Pk53C antibody, Pk?7 antibody, cdk antibody, cdk4 antibody, cdk4/6 antibody, dCdk4 antibody, l(2)05428 antibody, l(2)0671 antibody, l(2)k06503 antibody, l(2)s4639 antibody, l(2)sh0671 antibody, xcdk4 antibody, cyclin-dependent kinase 4 antibody, cyclin dependent kinase 4 antibody, Cyclin-dependent kinase 4 antibody, cyclin-dependent kinase 4 L homeolog antibody, cdk4 antibody, CDK4 antibody, Cdk4 antibody, cdk4.L antibody

背景

Cyclins, cyclin-dependent kinases (Cdks), and cyclin-dependent kinase inhibitors (CdkIs) are essential for cell-cycle control in eukaryotes (reviewed in 1). Cyclins, regulatory subunits, bind to cyclin-dependent kinases (Cdks), catalytic subunits, to form active cyclin-Cdk complexes. Cdk subunits by themselves are inactive and binding to a cyclin is required for their activity. Cyclins A, B1, D and E undergo periodic synthesis and degradation, thereby providing a mechanism to regulate Cdk activity throughout the cell cycle. Additionally, Cdk activity is further regulated by activating and inhibitory phosphorylations, and small proteins (p15, p16, p18, p19, p21 and p27), called CdkIs, that bind to cyclins, Cdks, or cyclin-Cdk complexes. Cdk4 was originally called PSK-J3,2 and following demonstration of its association with D-type cyclins, became known as Cdk4.2 D-type cyclins also associate with Cdks 2 and 5, although Cdk4 appears to be the most abundant partner. The D-type cyclins (D1, D2, and D3) are expressed in response to growth factors or mitogens, and rapidly degrade when mitogens are withdrawn. D cyclins appear to promote G0 to G1 transitions and the rate of G1 progression. For example, cyclin D-Cdk4 and cyclin D-Cdk6 complexes phosphorylate the retinoblastoma protein (Rb) which removes the G1 phase block caused by underphosphorylated Rb. Cdk4 has a molecular weight of ~33 kD.

分子量

33 kDa

途径

Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size