NFkB 抗体 (pSer529)
(3 references)-
- 抗原 See all NFkB 抗体
- NFkB (Nuclear Factor kappa B (NFkB))
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抗原表位
- pSer529
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NFkB antibody is un-conjugated
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应用范围
- Western Blotting (WB), Intracellular Staining (ICS)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Please refer to us for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Phosphorylated Human NF-kappaB p65 Peptide
- 克隆位点
- K10-895-12-50
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product NFkB Primary Antibody
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anti-Nuclear Factor kappa B (NFkB) (AA 42-367) antibody
NFkB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor kappa B (NFkB) (AA 805-892) antibodyNFkB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor kappa B (NFkB) (AA 352-439) antibodyNFkB 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor kappa B (NFkB) antibodyNFkB 适用: 人 CP unconjugated
anti-Nuclear Factor kappa B (NFkB) (AA 40-365) antibodyNFkB 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor kappa B (NFkB) (AA 47-372) antibodyNFkB 适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor kappa B (NFkB) (pSer536) antibodyNFkB 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB5212 unconjugated
anti-Nuclear Factor kappa B (NFkB) (pSer536) antibodyNFkB 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor kappa B (NFkB) (pSer536) antibodyNFkB 适用: 人 DB 宿主: 兔 Polyclonal RB22552 unconjugated
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- 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Interactions of NF-kappaB with chromatin: the art of being at the right place at the right time." in: Nature immunology, Vol. 6, Issue 5, pp. 439-45, (2005) (PubMed).
: "Control of lymphocyte development by nuclear factor-kappaB." in: Nature reviews. Immunology, Vol. 5, Issue 6, pp. 435-45, (2005) (PubMed).
: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, (2005) (PubMed).
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: "Interactions of NF-kappaB with chromatin: the art of being at the right place at the right time." in: Nature immunology, Vol. 6, Issue 5, pp. 439-45, (2005) (PubMed).
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- 抗原
- NFkB (Nuclear Factor kappa B (NFkB))
- 别名
- NF-kappa B (NFkB 产品)
- 别名
- EBP-1 antibody, KBF1 antibody, NF-kB1 antibody, NF-kappa-B antibody, NF-kappaB antibody, NFKB-p105 antibody, NFKB-p50 antibody, NFkappaB antibody, p105 antibody, p50 antibody, NF-kB antibody, NF-KB1 antibody, NF-kappaB1 antibody, p50/p105 antibody, nuclear factor kappa B subunit 1 antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 antibody, NFKB1 antibody, Nfkb1 antibody
- 背景
- Nuclear factor kB (NF-kB) is a ubiquitously expressed transcription factor that regulates the expression of 200-300 genes. It is crucial for basic cellular responses to stress and pathogens, such as proliferation, survival, development, and apoptosis. The most studied NF-kB complex consists of the p50 (also known as NF-kB1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor IkB and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkB. This complex prevents nuclear translocation and activity of NF-kB. In response to stimuli such as cytokines, LPS, DNA damage, and viral infections, IkB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkB/NF-kB complex, allowing the free heterodimeric NF-kB to translocate to the nucleus. Furthermore, optimal activation of NF-kB requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF-kB dimers bind to the kB sites within promoters and enhancers and function as transcriptional activators. The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of human NF-kB p65 subunit.
- 分子量
- 65 kDa
- 途径
- Ubiquitin Proteasome Pathway, S100 Proteins
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